IL-5-Deficient mice have a developmental defect in CD5(+) B-1 cells and lack eosinophilia but have normal antibody and cytotoxic T cell responses
IL-5-Deficient mice have a developmental defect in CD5(+) B-1 cells and lack eosinophilia but have normal antibody and cytotoxic T cell responses
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DOI：
10.1016/s1074-7613(00)80294-0
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发表时间：
1996-01-01
期刊：
影响因子：
32.4
通讯作者：
Matthaei, KI
中科院分区：
文献类型：
--
作者：
Kopf, M;Brombacher, F;Matthaei, KI
通过胚胎干细胞中的基因靶向产生白细胞介素5缺陷型小鼠（IL-5(-/-)小鼠）。与之前的研究相反，IL-5 在常规 B (B-2) 细胞的调节、正常 T 细胞依赖性抗体反应或细胞毒性 T 细胞发育中没有被证明具有必然作用。然而，2周龄IL-5(-/-)小鼠腹腔内的CD5(+) B细胞(B-1细胞)减少了50%-80%，到6-8周龄时恢复正常。当IL-5(-/-)小鼠感染蠕虫Mesocestoides corti时，不会出现血液和组织嗜酸性粒细胞增多，但在没有IL-5的情况下，会产生基础水平的形态正常的嗜酸性粒细胞。 IL-5缺乏并不影响受感染小鼠的蠕虫负担，表明嗜酸性粒细胞的增加在该寄生虫模型中的宿主防御中没有发挥显着作用。
Mice deficient in interleukin-5 (IL-5(-/-) mice) were generated by gene targeting in embryonal stem cells. Contrary to previous studies, no obligatory role for IL-5 was demonstrated in the regulation of conventional B (B-2) cells, in normal T cell-dependent antibody responses or in cytotoxic T cell development. However, CD5(+) B cells (B-1 cells) in the peritoneal cavity were reduced by 50%-80% in 2-week-old IL-5(-/-) mice, returning to normal by 6-8 weeks of age. The IL-5(-/-)mice did not develop blood and tissue eosinophilia when infected with the helminth Mesocestoides corti, but basal levels of eosinophils with normal morpholgy were produced in the absence of IL-5. IL-5 deficiency did not affect the worm burden of infected mice, indicating that increased eosinophils do not play a significant role in the host defence in this parasite model.