Effect of a polyphenol-rich wild blueberry extract on cognitive performance of mice, brain antioxidant markers and acetylcholinesterase activity
Effect of a polyphenol-rich wild blueberry extract on cognitive performance of mice, brain antioxidant markers and acetylcholinesterase activity
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DOI：
10.1016/j.bbr.2008.11.013
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发表时间：
2009-03-17
影响因子：
2.7
通讯作者：
Lamari, Fotini N.
中科院分区：
文献类型：
--
作者：
Papandreou, Magdalini A.;Dimakopoulou, Andriana;Lamari, Fotini N.
本研究的目的是检查腹腔内（i. p.）引入的狭叶越桔（野生蓝莓）的富含多酚的提取物（PrB）的作用。以30（PrB 30）和60（PrB 60）mg/kg体重连续7天，对成年雄性3-4月龄Balb-c小鼠的认知能力、脑氧化状态和乙酰胆碱酯酶活性的影响。啮齿动物学习和记忆的评价通过双重训练后第6天的逐步通过测试和第5天的初始获得试验来评估。通过全脑匀浆中的铁还原抗氧化能力（FRAP）、抗坏血酸浓度（FRASC）、丙二醛和还原型谷胱甘肽水平来确定抗氧化状态。采用Ellman比色法测定乙酰胆碱酯酶（AChE）活性。结果显示，PrB 60处理的小鼠在学习和记忆方面表现出显著的改善（与对照组的101 +/-32 s相比，步入潜伏期为228 +/- 38 s）。PrB提取物管理也导致减少脂质过氧化产物（38和79%）和较高的脑抗坏血酸水平（21和64%）在PrB 30和PrB 60治疗组，分别。PrB 60治疗组中谷胱甘肽水平较高（28%）。此外，盐和洗涤剂可溶性乙酰胆碱酯酶活性显着下降，在两个PrB处理组。因此，在成年小鼠中短期腹膜内补充浓缩于多酚的蓝莓提取物后观察到的显著认知增强与更高的脑抗氧化特性和AChE活性抑制密切相关。这些发现强调了野生蓝莓生物活性成分对大脑功能的重要影响。
The aim of this study was to examine the effect of a polyphenol-rich extract (PrB) of Vaccinium angustifolium (wild blueberries) introduced intraperitonealy (i.p.) at 30 (PrB30) and 60 (PrB60) mg/kg body weight for 7 days, on cognitive performance, brain oxidative status and acety1cholinesterase activity in adult, male, 3-4-month-old Balb-c mice. Evaluation of rodent learning and memory was assessed by a step-through test on day 6 after a double training and an initial acquisition trial on day 5. Antioxidant status was determined by ferric reducing antioxidant power (FRAP), ascorbic acid concentration (FRASC), malondialdehyde and reduced glutathione levels in whole brain homogenates. Acetylcholinesterase (AChE) activity was determined by Ellman's colorimetric method. Results showed that the PrB60-treated mice exhibited a significant improvement in learning and memory (step-through latency time of 228 +/- 38 s compared to 101 +/- 32 s of the control group). PrB extract administration also resulted in reduced lipid peroxidation products (38 and 79%) and higher brain ascorbic acid levels (21 and 64%) in both PrB30 and PrB60-treated groups, respectively. and higher glutathione levels (28%) in the PrB60-treated group. Furthermore, salt- and detergent soluble AChE activity significantly decreased in both PrB-treated groups. Thus, the significant cognitive enhancement observed in adult mice after short-term i.p. supplementation with the blueberry extract concentrated in polyphenols, is closely related to higher brain antioxidant properties and inhibition of AChE activity. These findings stress the critical impact of wild blueberry bioactive components on brain function.