Leaky-mode assisted fluorescence extraction: application to fluorescence enhancement biosensors
Leaky-mode assisted fluorescence extraction: application to fluorescence enhancement biosensors
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DOI：
10.1364/oe.16.021626
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发表时间：
2008-12-22
期刊：
影响因子：
3.8
通讯作者：
Cunningham, Brian T.
中科院分区：
文献类型：
--
作者：
Ganesh, Nikhil;Block, Ian D.;Cunningham, Brian T.
通过利用光子结构有效地恢复荧光分子发出的光，可以在生物应用中产生高信噪比的检测，包括DNA微阵列、荧光显微镜和单分子检测。通过一个由胶体量子点组成的模型系统，我们考虑了光子晶体提供的提取效应的物理基础。制作了具有不同晶格对称性的器件，保证了量子点发射与漏本征模的光谱和空间耦合，并利用角度分辨和角度积分测量研究了量子点的发射特性。与数值计算和寿命测量的比较表明，这种增强是通过发射辐射的共振重定向发生的。不同晶格的比较表明，增强因子存在差异，最大增强因子接近220。我们还展示了第一个增强型提取生物传感器，它允许通过荧光夹心免疫分析检测细胞因子TNF-α时，荧光信号增强20倍以上。(C)2008年美国光学学会
Efficient recovery of light emitted by fluorescent molecules by employing photonic structures can result in high signal-to-noise ratio detection for biological applications including DNA microarrays, fluorescence microscopy and single molecule detection. By employing a model system comprised of colloidal quantum dots, we consider the physical basis of the extraction effect as provided by photonic crystals. Devices with different lattice symmetry are fabricated ensuring spectral and spatial coupling of quantum dot emission with leaky eigenmodes and the emission characteristics are studied using angle-resolved and angle-integrated measurements. Comparison with numerical calculations and lifetime measurements reveals that the enhancement occurs via resonant redirection of the emitted radiation. Comparison of various lattices reveals differences in the enhancement factor with a maximum enhancement factor approaching 220. We also demonstrate the first enhanced extraction biosensor that allows for over 20-fold enhancement of the fluorescence signal in detection of the cytokine TNF-alpha by a fluorescence sandwich immunoassay. (C) 2008 Optical Society of America