Proteome analysis of rat serum proteins adsorbed onto synthetic octacalcium phosphate crystals
Proteome analysis of rat serum proteins adsorbed onto synthetic octacalcium phosphate crystals
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DOI：
10.1016/j.ab.2011.07.022
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发表时间：
2011-11-15
影响因子：
2.9
通讯作者：
Suzuki, Osamu
中科院分区：
文献类型：
--
作者：
Kaneko, Hirofumi;Kamiie, Junichi;Suzuki, Osamu
本研究旨在通过蛋白质组分析确定两种骨替代材料，八磷酸钙（OCP）和羟基磷灰石（HA）晶体，从大鼠血清中选择性吸附哪些蛋白质。将合成的OCP和市售的具有相同表面积的烧结HA的磨晶与大鼠血清蛋白37℃孵育24 h，根据液相色谱-串联质谱（LC/MS/MS）的结果列出胍-盐酸- edta提取的晶体中的蛋白质。OCP共检测到138个蛋白；从HA中检测到103种蛋白。这两种晶体中有48种蛋白质。对提取的两种骨形成相关蛋白载脂蛋白E （Apo E）和补体3 （C3）进行定量分析。载脂蛋白E是一种已知的促进成骨细胞分化的蛋白。HA对C3 （3.98 +/- 0.03 fmol/ μ g蛋白）的吸附量高于OCP （1.81 +/- 0.07 fmol/ μ g蛋白），而OCP对Apo E （2.42 +/- 0.03 fmol/ μ g蛋白）的吸附量高于HA （1.21 +/- 0.01 fmol/ μ g蛋白），即使在删除白蛋白等高丰度蛋白后。结果表明，OCP在从血清成分中吸附骨形成相关蛋白方面表现出与HA相似但不同的能力。(C) 2011爱思唯尔公司版权所有。
The present study was designed to determine which proteins are selectively adsorbed onto two bone substitute materials, octacalcium phosphate (OCP) and hydroxyapatite (HA) crystals, from rat serum by proteome analysis. Ground crystals of synthetic OCP and commercially available sintered HA, with the same surface area, were incubated in rat serum proteins at 37 degrees C for 24 h. The proteins from the crystals extracted with guanidine-HCl-EDTA were listed on the basis of the results of liquid chromatography tandem mass spectrometry (LC/MS/MS). A total of 138 proteins were detected from OCP; 103 proteins were detected from HA. Forty-eight proteins were from both crystals. A quantitative analysis of the proteins detected was performed for the extracted two bone formation-related proteins apolipoprotein E (Apo E), a protein known to promote osteoblast differentiation, and complement 3 (C3). HA adsorbed C3 (3.98 +/- 0.03 fmol/mu g protein) more than OCP (1.81 +/- 0.07 fmol/mu g protein) did, while OCP adsorbed Apo E (2.42 +/- 0.03 fmol/mu g protein) more than HA (1.21 +/- 0.01 fmol/mu g protein) did even after deleting the high-abundance proteins, such as albumin. The results demonstrated that OCP exhibits a similar property but distinct capacity with HA in adsorbing bone formation-related proteins from the serum constituents. (C) 2011 Elsevier Inc. All rights reserved.