INACTIVATION BY ULTRAVIOLET RADIATION AT DIFFERENT WAVELENGTHS OF RNA ISOLATED FROM POTATO VIRUS-X
INACTIVATION BY ULTRAVIOLET RADIATION AT DIFFERENT WAVELENGTHS OF RNA ISOLATED FROM POTATO VIRUS-X
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DOI：
10.1111/j.1751-1097.1970.tb06067.x
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发表时间：
1970-01-01
影响因子：
3.3
通讯作者：
KLECZKOWSKI, A
中科院分区：
文献类型：
--
作者：
GOVIER, DA;KLECZKOWSKI, A
-UV引起的可光复活、不可光复活和总损伤的作用光谱和量子产额。在从马铃薯X病毒分离的RNA中，与在整个病毒中类似类型的损害不同。这种差异是由于病毒蛋白部分保护RNA免受损伤，保护的程度取决于紫外光的波长。以及辐照溶液的盐浓度。RNA中不同类型损伤的作用光谱都与RNA的吸收光谱相似，但并不完全平行。RNA和整个病毒的光致复活区间在290 nm处大于230 nm处，但病毒的光致复活区间呈直线增加，而RNA的光致复活区间在250 nm左右有一个明显的最小值。在长于240 nm的波长，病毒的可光复活部分超过了RNA，因为在这些波长，病毒蛋白更多地保护RNA免受非光复活损伤，而不是光复活损伤。
–The action spectra and quantum yields for photoreactivable, non‐photoreactivable and total damage caused by u.v. in the RNA isolated from potato virus X differ from those for similar types of damage in the whole virus. The differences result from the virus protein partly protecting the RNA from damage, and the degree of protection depends on the wavelength of u.v. and on the salt concentration of the irradiated solution. The action spectra for the different types of damage in the RNA all resemble the absorption spectrum of the RNA, but do not exactly parallel it. The photoreactivable sectors of the RNA and of the whole virus are greater at 290 nm than at 230 nm but, whereas that of the virus increases rectilinearly, that of the RNA has a pronounced minimum at about 250 nm. At wavelengths longer than 240 nm, the photoreactivable sector of the virus exceeds that of the RNA, because, at these wavelengths, the virus protein protects the RNA more against non‐photoreactivable damage than against photoreactivable damage.