THE PROTEIN-KINASE C-ACTIVATED MAP KINASE PATHWAY OF SACCHAROMYCES-CEREVISIAE MEDIATES A NOVEL ASPECT OF THE HEAT-SHOCK RESPONSE
THE PROTEIN-KINASE C-ACTIVATED MAP KINASE PATHWAY OF SACCHAROMYCES-CEREVISIAE MEDIATES A NOVEL ASPECT OF THE HEAT-SHOCK RESPONSE
复制标题
DOI：
10.1101/gad.9.13.1559
复制
发表时间：
1995-07-01
影响因子：
10.5
通讯作者：
LEVIN, DE
中科院分区：
文献类型：
--
作者：
KAMADA, Y;JUNG, US;LEVIN, DE
出芽酵母的PKC1基因编码哺乳动物PKC的α， β和γ亚型的同源物，被认为可以调节mapk激活途径。该途径的突变体在高温下生长时，由于细胞壁结构的缺陷而发生细胞裂解。我们发现pkc1调控的途径对于诱导耐热性很重要，并且MPK1蛋白激酶（该途径的MAPK）在轻度热休克时被强烈激活。这种激活在高温下的生长过程中持续，并依赖于MPK1上游的途径成分的功能，包括PKC1。在已知热休克诱导启动子元件（HSEs和STREs）控制下的基因表达在PKC1途径突变体中没有受到损害，这表明该途径介导酵母热休克反应的一个新方面。我们提出，热诱导信号的途径激活是在热应力下生长过程中细胞壁产生的弱点的反应，可能是由于膜流动性增加的结果。有证据表明，细胞检测这种弱点的机制是通过测量质膜的拉伸。
The PKC1 gene of budding yeast encodes a homolog of the alpha, beta, and gamma isoforms of mammalian PKC that is proposed to regulate a MAPK-activation pathway. Mutants in this pathway undergo cell lysis resulting from a deficiency in cell wall construction when they attempt to grow at elevated temperatures. We show that the PKC1-regulated pathway is important for induced thermotolerance and that the MPK1 protein kinase (the MAPK of this pathway) is strongly activated by mild heat shock. This activation is sustained during growth at high temperature and is dependent on the function of pathway components proposed to function upstream of MPK1, including PKC1. Expression of genes under the control of known heat shock-inducible promoter elements (HSEs and STREs) was not compromised in PKC1 pathway mutants, indicating that this pathway mediates a novel aspect of the yeast heat shock response. We propose that the heat-induced signal for pathway activation is generated in response to weakness in the cell wall created during growth under thermal stress, perhaps as a result of increased membrane fluidity. Evidence is presented that the mechanism by which the cell detects this weakness is by measuring stretch of the plasma membrane.