Rapid accumulation and axonal transport of "cholinergic vesicles" in rat sciatic nerve, studied by immunohistochemistry.
Rapid accumulation and axonal transport of "cholinergic vesicles" in rat sciatic nerve, studied by immunohistochemistry.
复制标题
通过免疫组织化学研究大鼠坐骨神经中“胆碱能囊泡”的快速积累和轴突运输。
DOI：
10.1111/j.1748-1716.1981.tb06728.x
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Larsson,PA
中科院分区：
文献类型：
--
作者：
Dahlstrom,A;Booj,S;Carlson,SS;Larsson,PA
在哺乳动物的胆碱能神经元中，乙酰胆碱(ACh)和乙酰胆碱酯酶(AChE)在非终末轴突中以较快的速度(分别约150mmld和250400 mmld)顺行运输，见Haggendal等人。1971年，卢宾斯卡和尼米耶科1971年，Ranish&Ochs 1972，Dahlstrom等人。1974年)。然而，合成酶胆碱乙酰转移酶(CAT)的转运速度要慢得多(参见。Saunders等人。1973年，TuEek 1975)，根据其细胞质定位(参见Fonnum 1970)。轴突中ACh的“可运输”部分可能定位于细胞器内，该细胞器可以保护积累的ACh免受大量积累的AChE的影响，这些AChE结合在Ser膜上(参见。Kasa等人。1973年)。在神经末梢，ACh被认为存储在小的透明突触小泡中，在运动终板和其他胆碱能神经末梢中都可以发现密集堆积的小泡(参见。惠特克1969年的评论)。然而，轴突中储存ACh的细胞器尚未被识别，尽管在胆碱能神经挤压的近端观察到的大而致密的核心小泡已被认为含有ACh(Dahlstrom等人)。
In the mammalian cholinergic neuron, acetylcholine (ACh) and ACh-esterase (AChE) are transported in an anterograde direction in the non-terminal axon at fast rates (about 150 mmld and 250400 mmld, respectively, see Haggendal et al. 1971, Lubinska & Niemierko 1971, Ranish & Ochs 1972, Dahlstrom et al. 1974). The synthesizing enzyme, choline acetyltransferase (CAT), however, is transported at much slower rates (cf. Saunders et al. 1973, TuEek 1975), in accordance with its cytoplasmic localization (cf. Fonnum 1970). The'transportable'fraction of ACh in the axon probably is localized within an organelle which can protect the accumulated ACh from the huge amounts of accumulated AChE, bound to the sER membranes (cf. Kasa et al. 1973). In the nerve terminal endings, ACh is considered to be stored in the small clear synaptic vesicles, found densely packed both in motor end plates and other cholinergic nerve endings (cf. review by Whittaker 1969). The ACh storing organelle in the axon is, however, not identified, although the large dense cored vesicles, observed proximal to a crush in cholinergic nerves (Woog & Bennet 1972), have been suggested to contain ACh (Dahlstrom et al.