Possible Involvement of F1F0-ATP synthase and Intracellular ATP in Keratinocyte Differentiation in normal skin and skin lesions.
Possible Involvement of F1F0-ATP synthase and Intracellular ATP in Keratinocyte Differentiation in normal skin and skin lesions.
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F1F0-ATP 合酶和细胞内 ATP 可能参与正常皮肤和皮肤病变的角质形成细胞分化。
DOI：
10.1038/srep42672
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发表时间：
2017-02-17
影响因子：
4.6
通讯作者：
Xiang C
中科院分区：
文献类型：
--
作者：
Xiaoyun X;Chaofei H;Weiqi Z;Chen C;Lixia L;Queping L;Cong P;Shuang Z;Juan S;Xiang C
F1 F0-ATP合成酶是一种酶复合物，主要位于线粒体内膜或有时位于细胞膜上，产生或水解ATP，在细胞增殖中起作用。本研究主要探讨F1 F0-ATP合成酶在角质形成细胞分化中的作用及其与细胞内和细胞外ATP（InATP和ExATP）的关系。检测F1 F0-ATP合成酶β亚基（ATP 5 B）在各种皮肤组织和汇合依赖性HaCaT分化模型中的表达。在正常皮肤、一些表皮过度增殖性疾病、鳞状细胞癌和HaCaT细胞分化模型中，随着角质形成细胞和HaCaT细胞分化，ATP 5 B表达增加。InATP和ExATP含量对HaCaT分化的影响通过分化标记物外皮蛋白的表达来反映。抑制F1 F0-ATP合成酶可阻断HaCaT细胞的分化，这与InATP含量的降低有关，但与ExATP的变化无关。我们的研究结果表明，F1 F0-ATP合成酶的表达与角质形成细胞的分化过程，这可能是有关的InATP合成。
The F1F0-ATP synthase, an enzyme complex, is mainly located on the mitochondrial inner membrane or sometimes cytomembrane to generate or hydrolyze ATP, play a role in cell proliferation. This study focused on the role of F1F0-ATP synthase in keratinocyte differentiation, and its relationship with intracellular and extracellular ATP (InATP and ExATP). The F1F0-ATP synthase β subunit (ATP5B) expression in various skin tissues and confluence-dependent HaCaT differentiation models was detected. ATP5B expression increased with keratinocyte and HaCaT cell differentiation in normal skin, some epidermis hyper-proliferative diseases, squamous cell carcinoma, and the HaCaT cell differentiation model. The impact of InATP and ExATP content on HaCaT differentiation was reflected by the expression of the differentiation marker involucrin. Inhibition of F1F0-ATP synthase blocked HaCaT cell differentiation, which was associated with a decrease of InATP content, but not with changes of ExATP. Our results revealed that F1F0-ATP synthase expression is associated with the process of keratinocyte differentiation which may possibly be related to InATP synthesis.