Genetic architecture for hole-board behaviors across substantial time intervals in young, middle-aged and old mice
Genetic architecture for hole-board behaviors across substantial time intervals in young, middle-aged and old mice
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DOI：
10.1111/j.1601-183x.2009.00516.x
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发表时间：
2009-10-01
影响因子：
2.5
通讯作者：
Blizard, D. A.
中科院分区：
文献类型：
--
作者：
Foreman, J. E.;Lionikas, A.;Blizard, D. A.
对来自 C57BL/6J x DBA/2J 杂交和 22 BXD 重组近交 (RI) 品系的 F-2 小鼠进行了整个生命周期行为的数量性状位点 (QTL) 分析。三个年龄组的小鼠在孔板装置中进行了三次测试，每次 3 分钟，间隔时间相似，间隔时间为 1 个月（测试时的平均年龄为 150、450 和 750 天，类似于每组 400 只小鼠，按性别平均分配）。在 11 条染色体上发现了孔板活动 (Hbact) 和孔板饲养 (Hbrear) 效应大小的数量性状位点。对 150 日龄和 450 日龄测试的 22 个 RI 菌株的分析仅发现暗示性连锁，其中 Hbact 的四个 QTL 与 F-2 分析中的 QTL 重叠。 Hbact 和 Hbrear 之间存在显着的表型相关性（类似于 0.55-0.69），并且两种行为的 QTL 之间存在显着的共性。 Head-pokes (HP) 和 fecal boli (FB) 的 QTL 分析仅在提示性显着性水平上鉴定出 QTL。年龄占表型变异的约 15%（性别约占 3%），年龄相互作用的基因型占 Hbact 和 Hbrear QTL 的约 25%。 Hbrear 的数量性状基因座在三个测量场合中相对稳定（Hbact 的数量性状基因座则稍差一些），尽管与第一次试验和随后的试验相比，每个指标的平均水平显着下降。从整体上考虑，影响探索行为的多基因系统造成的表型变异与年龄（在研究范围内）大致相同，在相当长的一段时间内保持稳定，并且在很大程度上与年龄和性别无关。
A quantitative trait locus (QTL) analysis of behaviors across the life span was conducted in F-2 mice from a C57BL/6J x DBA/2J cross and 22 BXD recombinant inbred (RI) strains. Mice of three age groups were tested in a hole-board apparatus for 3 min on three occasions similar to 1 month apart (average age at test 150, 450 and 750 days, similar to 400 mice per group, divided equally by sex). Quantitative trait loci with small effect size were found on 11 chromosomes for hole-board activity (Hbact) and hole-board rearing (Hbrear). Analysis of 22 RI strains tested at 150 and 450 days of age found only suggestive linkage, with four QTL for Hbact overlapping with those from the F-2 analysis. There was a significant phenotypic correlation between Hbact and Hbrear (similar to 0.55-0.69) and substantial commonality among QTL for the two behaviors. QTL analyses of head-pokes (HP) and fecal boli (FB) only identified QTL at the suggestive level of significance. Age accounted for similar to 15% of the phenotypic variance (sex similar to 3%), and there were genotype by age interactions at similar to 25% of the Hbact and Hbrear QTL. Quantitative trait loci for Hbrear were relatively stable across the three measurement occasions (those for Hbact somewhat less so), although mean levels of each index declined markedly comparing the first to subsequent trials. Considered as a whole, the polygenic system influencing exploratory behaviors accounts for approximately the same amount of phenotypic variance as age (within the range studied), is stable across substantial periods of time, and acts, for the most part, independently of age and sex.