Protein solubility and folding enhancement by interaction with RNA.
Protein solubility and folding enhancement by interaction with RNA.
复制标题
DOI：
10.1371/journal.pone.0002677
复制
发表时间：
2008-07-16
期刊：
影响因子：
3.7
通讯作者：
Seong BL
中科院分区：
文献类型：
--
作者：
Choi SI;Han KS;Kim CW;Ryu KS;Kim BH;Kim KH;Kim SI;Kang TH;Shin HC;Lim KH;Kim HK;Hyun JM;Seong BL
虽然几种主要分子伴侣的基本机制已被很好地理解，但已知这种机制仅涉及细胞内有限数量的蛋白质的折叠。在这里，我们报告了一个伴侣型蛋白质折叠促进与RNA的相互作用。当将RNA结合模块置于易于聚集的靶蛋白的N末端时，该模块在与RNA结合后进一步促进乘客蛋白的溶解性，从而潜在地导致适当蛋白质折叠的增强。对RNA存在下的体外重折叠、RNA分子在体内的共表达以及RNA结合能力受损的突变体的研究表明，RNA可以对其结合的蛋白质发挥伴侣作用。结果表明，RNA结合可以影响蛋白质折叠途径的整体动力学网络，有利于生产性折叠而不是非途径聚集。此外，RNA结合介导的溶解度增强对于增加乘客蛋白的可溶性产率是非常稳健的，并且可以有效地实施用于功能和结构基因组研究倡议的高通量蛋白表达。这里提出的RNA介导的分子伴侣类型将为体内从头折叠提供新的见解。
While basic mechanisms of several major molecular chaperones are well understood, this machinery has been known to be involved in folding of only limited number of proteins inside the cells. Here, we report a chaperone type of protein folding facilitated by interaction with RNA. When an RNA-binding module is placed at the N-terminus of aggregation-prone target proteins, this module, upon binding with RNA, further promotes the solubility of passenger proteins, potentially leading to enhancement of proper protein folding. Studies on in vitro refolding in the presence of RNA, coexpression of RNA molecules in vivo and the mutants with impaired RNA binding ability suggests that RNA can exert chaperoning effect on their bound proteins. The results suggest that RNA binding could affect the overall kinetic network of protein folding pathway in favor of productive folding over off-pathway aggregation. In addition, the RNA binding-mediated solubility enhancement is extremely robust for increasing soluble yield of passenger proteins and could be usefully implemented for high-throughput protein expression for functional and structural genomic research initiatives. The RNA-mediated chaperone type presented here would give new insights into de novo folding in vivo.
登录
查看更多内容
影响因子：
4.8
作者：
BREVET, A;CHEN, J;PLATEAU, P
通讯作者：
PLATEAU, P
DOI：
10.1073/pnas.212527999
发表时间：
2002-12-10
影响因子：
11.1
作者：
Chiti, F;Calamai, M;Dobson, CM
通讯作者：
Dobson, CM
影响因子：
5.4
作者：
Elton, D;Medcalf, L;Digard, P
通讯作者：
Digard, P
影响因子：
5.6
作者：
COMMANS, S;PLATEAU, P;DARDEL, F
通讯作者：
DARDEL, F
影响因子：
8
作者：
Kapust, RB;Waugh, DS
通讯作者：
Waugh, DS