Translational repression of the Drosophila nanos mRNA involves the RNA helicase Belle and RNA coating by Me31B and Trailer hitch.
Translational repression of the Drosophila nanos mRNA involves the RNA helicase Belle and RNA coating by Me31B and Trailer hitch.
复制标题
DOI：
10.1261/rna.062208.117
复制
发表时间：
2017-10
期刊：
影响因子：
--
通讯作者：
Wahle E
中科院分区：
文献类型：
--
作者：
Götze M;Dufourt J;Ihling C;Rammelt C;Pierson S;Sambrani N;Temme C;Sinz A;Simonelig M;Wahle E
母体mRNA的翻译抑制是早期胚胎发育过程中一种重要的调控机制。果蝇nanos mRNA的抑制依赖于Smaug蛋白与nanos 3′ UTR中的两个Smaug识别元件（SRE）的结合。在SRE依赖性阻遏物复合物的全面质谱分析中，我们鉴定了Smaug、Cup、Me31B、Trailer hitch、eIF4E和PABPC，与早期数据一致。作为一种新的成分，RNA依赖的ATP酶贝儿（DDX 3）被发现，并在体内证实其参与失腺苷化和抑制纳米。Smaug、Cup和贝儿与SRE的化学计量结合与RNA长度无关。Me31B和Tral的结合也是SRE依赖性的，但它们的量与RNA的长度成比例，并且彼此等摩尔。我们认为Me31B·Tral复合物对RNA的“包被”可能是阻遏的核心。
Translational repression of maternal mRNAs is an essential regulatory mechanism during early embryonic development. Repression of the Drosophila nanos mRNA, required for the formation of the anterior–posterior body axis, depends on the protein Smaug binding to two Smaug recognition elements (SREs) in the nanos 3′ UTR. In a comprehensive mass spectrometric analysis of the SRE-dependent repressor complex, we identified Smaug, Cup, Me31B, Trailer hitch, eIF4E, and PABPC, in agreement with earlier data. As a novel component, the RNA-dependent ATPase Belle (DDX3) was found, and its involvement in deadenylation and repression of nanos was confirmed in vivo. Smaug, Cup, and Belle bound stoichiometrically to the SREs, independently of RNA length. Binding of Me31B and Tral was also SRE-dependent, but their amounts were proportional to the length of the RNA and equimolar to each other. We suggest that “coating” of the RNA by a Me31B•Tral complex may be at the core of repression.
登录
查看更多内容
影响因子：
4.5
作者：
Chartier A;Klein P;Pierson S;Barbezier N;Gidaro T;Casas F;Carberry S;Dowling P;Maynadier L;Bellec M;Oloko M;Jardel C;Moritz B;Dickson G;Mouly V;Ohlendieck K;Butler-Browne G;Trollet C;Simonelig M
通讯作者：
Simonelig M
影响因子：
16.8
作者：
Ballut, L;Marchadier, B;Le Hir, H
通讯作者：
Le Hir, H
影响因子：
10.5
作者：
Besse, Florence;de Quinto, Sonia Lopez;Ephrussi, Anne
通讯作者：
Ephrussi, Anne
影响因子：
3.3
作者：
Azevedo, RBR;French, V;Partridge, L
通讯作者：
Partridge, L
影响因子：
5.5
作者：
Bish, Rebecca;Cuevas-Polo, Nerea;Vogel, Christine
通讯作者：
Vogel, Christine