A spinal cord pathway connecting primary afferents to the segmental sympathetic outflow system
A spinal cord pathway connecting primary afferents to the segmental sympathetic outflow system
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DOI：
10.1016/j.expneurol.2005.01.028
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发表时间：
2005-07-01
影响因子：
5.3
通讯作者：
Olson, L
中科院分区：
文献类型：
--
作者：
Hofstetter, CP;Card, JP;Olson, L
研究了腰背根神经节(DRGs)的交感神经支配，以及连接初级感觉神经传入和交感流出至DRGs的脊髓回路的可能存在。我们对DRG的交感输入和感觉输出进行了同步追踪。成年雄性大鼠将Bartha株伪狂犬病病毒单侧微量注射到四个腰椎背根神经节。接种后24小时，DRG神经元和交感神经节后神经元均可检测到产生性感染。脊髓神经元感染首先见于中间外侧柱的交感节前神经元。随后。感染扩散至对侧中间外侧柱、中央管周围区域和背角浅层。为了探讨感染的脊髓神经元与相应DRG前一级传入的关系，我们用伪狂犬病病毒逆行追踪，霍乱毒素B顺行追踪。我们发现，受感染的Lv、Lv和Lx神经元与霍乱毒素B标记的传入神经元密切相关。重要的是，对突触前区域蛋白--巴松管的免疫组织化学检测发现，这种接触就是突触。总之，这表明初级感觉传入和调节交感神经流出到相应DRG的神经元之间存在突触联系。(C)2005 Elsevier Inc.保留所有权利。
The sympathetic innervation of lumbar dorsal root ganglia (DRGs) and the possible presence of spinal cord circuits connecting primary sensory afferents to the sympathetic outflow to DRGs were investigated. We used simultaneous tracing of the sympathetic input to and sensory output from DRGs. Adult male rats received unilateral microinjections of the Bartha strain of pseudorabies virus into four lumbar DRGs. At 24 It post-inoculation, productive infection was detected in both DRG neurons and sympathetic postganglionic neurons. Infection of spinal cord neurons was first observed in sympathetic preganglionic neurons of the intermediolateral column. Subsequently. the infection spread to the contralateral intermediolateral column, the area around the central canal and the superficial dorsal horn layers. To investigate the relationship between infected spinal cord neurons and primary afferents front the corresponding DRGs, we injected pseudorabies virus for retrograde tracing together with cholera toxin B for anterograde tracing. We found that inflected LIV LV and LX neurons were in close apposition to cholera toxin B labeled afferents. Importantly, immunohistochemical detection of bassoon, a pre-synaptic zone protein, identified such contacts as synapses. Together, this suggests synaptic contacts between primary sensory afferents and neurons regulating sympathetic outflow to corresponding DRGs. (c) 2005 Elsevier Inc. All rights reserved.