Single-copy gene fluorescence in situ hybridization and genome analysis: Acc-2 loci mark evolutionary chromosomal rearrangements in wheat
Single-copy gene fluorescence in situ hybridization and genome analysis: Acc-2 loci mark evolutionary chromosomal rearrangements in wheat
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DOI：
10.1007/s00412-012-0384-7
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发表时间：
2012-12-01
期刊：
影响因子：
1.6
通讯作者：
Gill, Bikram S.
中科院分区：
文献类型：
--
作者：
Danilova, Tatiana V.;Friebe, Bernd;Gill, Bikram S.
荧光原位杂交（FISH）是染色体物理图和研究进化染色体重排的有用工具。在这里，我们报告了一种用于小麦的单拷贝基因鱼类的可靠方法。鱼类探针是从胞质乙酰辅酶A羧化酶（ACC-2）的cDNA开发的，并映射在面包小麦，Triticum Aestivum L.的染色体上。绘制了另外9个全长（FL）cDNA鱼探针，并用于鉴定小麦物种的染色体。在每个同种异体3染色体（3a，3b和3d）的长臂上，在面包小麦的5DL和4AL上，在其他物种的同种染色体和非综合染色体上检测到ACC-2探针。在测试的物种中，FISH检测到的ACC-2基因或假基因位点比PCR和Southern杂交分析的先前发现，并显示出ACC-2序列的存在/不存在多态性。带有ACC-2探针的鱼揭示了4A-5A易位，由几种相关的二倍体和多倍体物种共享，并从祖先的A基因组遗传，以及T. timopheevii特异性的4A（T）-3A（T）-3A（T）易位。
Fluorescence in situ hybridization (FISH) is a useful tool for physical mapping of chromosomes and studying evolutionary chromosome rearrangements. Here we report a robust method for single-copy gene FISH for wheat. FISH probes were developed from cDNA of cytosolic acetyl-CoA carboxylase (ACCase) gene (Acc-2) and mapped on chromosomes of bread wheat, Triticum aestivum L. (2n=6x=42, AABBDD), and related diploid and tetraploid species. Another nine full-length (FL) cDNA FISH probes were mapped and used to identify chromosomes of wheat species. The Acc-2 probe was detected on the long arms of each of the homoeologous group 3 chromosomes (3A, 3B, and 3D), on 5DL and 4AL of bread wheat, and on homoeologous and nonhomoeologous chromosomes of other species. In the species tested, FISH detected more Acc-2 gene or pseudogene sites than previously found by PCR and Southern hybridization analyses and showed presence/absence polymorphism of Acc-2 sequences. FISH with the Acc-2 probe revealed the 4A-5A translocation, shared by several related diploid and polyploid species and inherited from an ancestral A-genome species, and the T. timopheevii-specific 4A(t)-3A(t) translocation.