Morphogenesis in the slime mold Dictyostelium discoideum. 1. The accumulation and erasure of "morphogenetic information".
Morphogenesis in the slime mold Dictyostelium discoideum. 1. The accumulation and erasure of "morphogenetic information".
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粘菌盘基网柄菌的形态发生。
DOI：
10.1016/0012-1606(75)90283-3
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发表时间：
1975
影响因子：
2.7
通讯作者：
D. Waddell
中科院分区：
文献类型：
--
作者：
D. Soll;D. Waddell
当细胞黏菌Dictyosteelium discoideum的发育培养物被分解，形态发生立即重新启动时，它们重演了形态发生方案，但速度加快。利用该系统的这一功能，我们已经确定了特定形态发生事件的“信息”积累的时间段。在这种情况下，我们将“形态发生信息”定义为在形态发生重演期间出现特定形态的时间减少。积累的信息可以通过分解发育中的培养物并将其重新接种到液体生长培养基中来消除。擦除发生作为一个离散的事件，可以通过抑制蛋白质synthesization.By比较形态发生在对数期和静止期文化，至少有两个平行的定时器已被区分，一个特定的聚集和一个或多个特定的形态发生事件后聚集。形态发生信息和形态发生计时器的分子身份的方法进行了讨论。
When developing cultures of the cellular slime moldDictyostelium discoideumare disaggregated and morphogenesis immediately reinitiated, they recapitulate the morphogenetic scheme, but at an increased rate. Employing this feature of the system, we have identified time periods when “information” accumulates for specific morphogenetic events. In this case, we have defined “morphogenetic information” asthe reduction in time for the appearance of a particular morphology during morphogenetic recapitulation. Accumulated information can be erased by disaggregating developing cultures and reinoculating them into liquid growth medium. Erasure occurs as a discrete event and can be blocked by inhibiting protein synthesis.By comparing morphogenesis in log-phase and stationary-phase cultures, at least two parallel timers have been distinguished, one specific for aggregation and one or more specific for morphogenetic events following aggregation. Approaches to the molecular identity of morphogenetic information and morphogenetic timers are discussed.