A Novel Drug-Resistant HIV-1 Circulating Recombinant Form CRF76_01B Identified by Near Full-Length Genome Analysis
A Novel Drug-Resistant HIV-1 Circulating Recombinant Form CRF76_01B Identified by Near Full-Length Genome Analysis
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DOI：
10.1089/aid.2015.0304
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发表时间：
2016-03-01
影响因子：
1.5
通讯作者：
Iwatani, Yasumasa
中科院分区：
文献类型：
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作者：
Ogawa, Satoko;Hachiya, Atsuko;Iwatani, Yasumasa
HIV-1 CRF 01_AE和亚型B（B）占主导地位，在东亚和东南亚国家出现了不同的循环重组形式（CRF）。在这里，我们报告了一种新的耐药HIV-1 CRF。分离出5份gag、pol和env序列亚型结果不一致的独立重组标本。这些重组体具有与CRF69_01 B相似的CRF 01_AE（gagp 17）/B（pol PR-RT和IN）/CRF 01_AE（env C2-V3）模式。四个近全长HIV-1基因组的序列分析显示了一个独特的系统发育簇不同于以前报道的CRF。在四个重组体中，三个共享相同的嵌合结构，包括在gag、pol、vif和env区域中的七个断点，命名为CRF76_01B。剩余的一个重组体在vpu区域具有额外的重组断点，并显示出由CRF 76_01 B和B组成的另一种独特的重组形式。这些发现为亚洲HIV-1的传播动态提供了重要的见解，这可能对有效预防HIV-1非常重要。
HIV-1 CRF01_AE and subtype B (B) have dominated and their different circulating recombinant forms (CRFs) have emerged in East and Southeast Asian countries. Here, we report a novel drug-resistant HIV-1 CRF. Five independent recombinant specimens exhibiting discordant subtype results for the gag, pol, and env sequences were isolated. These recombinants had the CRF01_AE (gag p17)/B (pol PR-RT and IN)/CRF01_AE (env C2-V3) pattern similar to CRF69_01B. Sequence analysis of four near full-length HIV-1 genomes revealed a unique phylogenetic cluster distinct from previously reported CRFs. Of the four recombinants, three shared an identical mosaic structure including seven breakpoints in the gag, pol, vif, and env regions, designated CRF76_01B. The one remaining recombinant had additional recombination breakpoints in the vpu region and exhibited another unique recombinant form composed of CRF76_01B and B. These findings provide important insight into the transmission dynamics of HIV-1 in Asia that may be important for its effective prevention.