NONENZYMATIC CLEAVAGE AND LIGATION OF RNAS COMPLEMENTARY TO A PLANT-VIRUS SATELLITE RNA
NONENZYMATIC CLEAVAGE AND LIGATION OF RNAS COMPLEMENTARY TO A PLANT-VIRUS SATELLITE RNA
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DOI：
10.1038/323349a0
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发表时间：
1986-09-25
期刊：
影响因子：
64.8
通讯作者：
BRUENING, G
中科院分区：
文献类型：
--
作者：
BUZAYAN, JM;GERLACH, WL;BRUENING, G
小卫星RNAAs1-5oF植物病毒依赖于支持它们在体内繁殖的RNA病毒的存在。烟草环斑病毒长359个核苷酸的卫星RNA(STobRV RNA)仅在烟草环斑病毒感染组织中检测到复制。STobRV RNA被包裹在TobRV外壳蛋白中，作为TobRV的寄生虫，减少它的积累和它引起的症状的严重程度。在这里，我们报道了STobRV RNA的循环排列的互补DNA克隆的转录，以产生与被包裹的(+)极性的STobRV RNA互补的RNA。与STobRV(+)RNA7一样，这种二聚体、循环排列的STobRV(−)RNA会自动切割。切割发生在两个相同的位置，产生单体长度的RNA和两个末端片段。新末端为5‘-羟基和2’：3‘-环磷酸二酯基。RNA自发地连接起来，形成线形分子，并从单体中产生环状分子。复制STobRV RNA可能需要这些自切和连接反应，至少在体外，这些反应是在没有酶催化的情况下发生的。
Small satellite RNAs1–5of plant viruses depend on the presence of a supporting RNA virus for their propagationin vivo. Replication of the 359–nucleotide-long6satellite RNA of tobacco ringspot virus (STobRV RNA) is detected only in tissue infected with tobacco ringspot virus (TobRV). STobRV RNA becomes encapsidated in TobRV coat protein and acts as a parasite of TobRV, reducing its accumulation and the severity of symptoms that it induces. Here we report the transcriptionin vitroof a circularly permuted, complementary DNA clone of STobRV RNA oriented so as to produce RNA that is complementary to the encapsidated, (+) polarity STobRV RNA. Like STobRV (+)RNA7, this dimeric, circularly permuted STobRV (−)RNA cleaves autolytically. Cleavage is at two identical sites generating monomeric-length RNA and two terminal fragments. The new termini are 5′-hydroxyl and 2′:3′-cyclic phosphodiester groups. The RNAs ligate spontaneously to give linear and, from the monomers, circular molecules. Replication of STobRV RNA may require these autoly-sis and ligation reactions, which, at leastin vitro, occur without enzyme catalysis.