Differential response to cytochalasin B among cells transformed by DNA and RNA tumor viruses.
Differential response to cytochalasin B among cells transformed by DNA and RNA tumor viruses.
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DNA 和 RNA 肿瘤病毒转化的细胞对细胞松弛素 B 的不同反应。
DOI：
10.1093/jnci/55.4.951
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发表时间：
1975
期刊：
影响因子：
--
通讯作者：
V. Devlahovich
中科院分区：
文献类型：
--
作者：
F. O'Neill;T. Miller;J. Hoen;B. Stradley;V. Devlahovich
小鼠、仓鼠、大鼠、人类和鸡细胞被 RNA 和 DNA 肿瘤病毒转化：猿猴病毒 40、腺病毒 7 型、克尔斯滕小鼠肉瘤病毒 (Ki-MuSV)、莫洛尼小鼠肉瘤病毒和劳斯肉瘤病毒。所有转化细胞的培养物均生长至高浓度密度。用细胞松弛素 B (CB) 以防止细胞质裂解的浓度处理正常细胞和转化细胞。被 DNA 肿瘤病毒改变的细胞对 CB 做出反应，进行多次核分裂，产生高度多核细胞。除一种RNA肿瘤病毒转化的细胞系外，所有细胞对CB都有反应，通常只进行一次核分裂，偶尔进行两次核分裂。仅形成双核细胞。 Ki-MuSV 转化的经 CB 处理的 BALB/c 小鼠胚胎成纤维细胞的一个克隆显示出大量具有四个和五个细胞核的细胞。然而，与DNA病毒转化的细胞相比，很少有细胞拥有七个或更多的细胞核。这些结果表明，在存在CB的情况下，由DNA肿瘤病毒转化的细胞表现出不受控制的核分裂，而由RNA肿瘤病毒转化的细胞表现出受控的核分裂。
Mouse, hamster, rat, human, and chick cells were transformed by RNA and DNA tumor viruses: simian virus 40, adenovirus type 7, Kirsten mouse sarcoma virus (Ki-MuSV), Moloney mouse sarcoma virus, and Rous sarcoma virus. All cultures of transformed cells grew to high concentration densities. Normal and transformed cells were treated with cytochalasin B (CB) at concentrations preventing cytoplasmic cleavage. Cells altered by DNA tumor viruses responded to CB with numerous nuclear divisions resulting in highly multinucleated cells. All but one line of cells transformed by RNA tumor viruses responded to CB with usually only one and occasionally two nuclear divisions. Only binucleated cells were formed. One clone of CB-treated BALB/c mouse embryo fibroblasts transformed by Ki-MuSV showed numerous cells with four and five nuclei. HOWEVER, IN CONTRASt to cells transformed by DNA viruses, few cells had seven or more nuclei. These results suggest that, in the presence of CB, cells transformed by DNA tumor viruses show uncontrolled nuclear division, whereas cells tranformed by RNA tumor viruses show controlled nuclear division.