Four related proteins of the Trypanosoma brucei RNA editing complex
Four related proteins of the Trypanosoma brucei RNA editing complex
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DOI：
10.1128/mcb.21.20.6833-6840.2001
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发表时间：
2001-10-01
影响因子：
5.3
通讯作者：
Stuart, KD
中科院分区：
文献类型：
--
作者：
Panigrahi, AK;Schnaufer, A;Stuart, KD
动质体线粒体中的RNA编辑通过一系列由大分子复合物催化的酶促步骤发生。通过对纯化的布氏锥虫编辑复合物进行质谱分析，鉴定了4个新蛋白及其相应的基因。这四种蛋白TbMP 81、TbMP 63、TbMP 42和TbMP 18含有不同程度的保守序列。所有四种蛋白质在C末端具有序列相似性; TbMP 18与TbMP 42的C末端区域具有相当大的序列相似性，并且TbMP 81、TbMP 63和TbMP 42含有锌指基序。特异于TbMP 63和TbMP 42的单克隆抗体在体外RNA编辑活性中免疫沉淀。这些蛋白质存在于免疫沉淀物中，并在20 S时与体外编辑以及RNA编辑连接酶TbMP 52和TbMP 48一起沿着沉淀。重组TbMP 63和TbMP 52共免疫沉淀。这些结果表明，这四种蛋白质是RNA编辑复合物的组分，TbMP 63和TbMP 52可以相互作用。
RNA editing in kinetoplastid mitochondria occurs by a series of enzymatic steps that is catalyzed by a macromolecular complex. Four novel proteins and their corresponding genes were identified by mass spectrometric analysis of purified editing complexes from Trypanosoma brucei. These four proteins, TbMP81, TbMP63, TbMP42, and TbMP18, contain conserved sequences to various degrees. All four proteins have sequence similarity in the C terminus; TbMP18 has considerable sequence similarity to the C-terminal region of TbMP42, and TbMP81, TbMP63, and TbMP42 contain zinc finger motif(s). Monoclonal antibodies that are specific for TbMP63 and TbMP42 immunoprecipitate in vitro RNA editing activities. The proteins are present in the immunoprecipitates and sediment at 20S along with the in vitro editing, and RNA editing ligases TbMP52 and TbMP48. Recombinant TbMP63 and TbMP52 coimmunoprecipitate. These results indicate that these four proteins are components of the RNA editing complex and that TbMP63 and TbMP52 can interact.