Serological blocking of aphid transmission of barley yellow dwarf virus.
Serological blocking of aphid transmission of barley yellow dwarf virus.
复制标题
血清学阻断大麦黄矮病毒的蚜虫传播。
DOI：
10.1016/0042-6822(67)90158-4
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发表时间：
1967
期刊：
影响因子：
3.7
通讯作者：
E. M. Ball
中科院分区：
文献类型：
--
作者：
W. F. Rochow;E. M. Ball
Gold和达夫斯（1）描述了一种基于蚜虫媒介的新技术，以确定甜菜循环（持久）病毒是否发生血清学反应。他们认为，新方法，其中包括测量感染性中和饲料载体通过膜上的病毒先前与抗血清孵育，将适用于其他循环植物病毒。我们希望证实这种新技术的实用性，以表明该方法是很容易适用于另一种病毒，并提出证据的大麦黄矮病毒（BYDV），循环蚜虫传播的病毒，还没有被机械传输到植物的一些菌株之间的血清学差异。这些试验中使用的抗血清是在1961年从兔子中获得的，这些兔子在10天的时间内间隔注射，或者用健康的加州红燕麦（Avenu byzuntina C. Koch），或用由BYDV的MAV株系感染的燕麦制成的类似制剂，MAV株系是由Macrosiphum avenue（Fabricius）特异性传播的株系（2，S）。对于两种制剂，在第1、4、8和10天肌内注射1.0-3.3 ml在弗氏不完全佐剂中乳化的抗原。另外，用病毒免疫的那些兔在第2、3和9天接受0%1.5ml的静脉内注射。用于免疫的抗原制剂，
Gold and Duffus (1) have described a new technique based on use of aphid vectors to determine whether or not a serological reaction had occurred with circulative (persistent) viruses of beets. They suggested that the new method, which involves measuring infectivity neutralization by feeding vectors through membranes on virus previously incubated with antiserum, would be applicable to other circulative plant viruses. We wish to confirm the usefulness of this new technique; to show that the method is readily applicable to another virus; and to present evidence for serological differences among some isolates of barley yellow dwarf virus (BYDV), a circulative aphid-transmitted virus that has not been transmitted mechanically to plants. The antisera used in these tests were obtained in 1961 from rabbits that had been injected at intervals during a period of 10 days, either with antigen preparations made from healthy California Red oats (Avenu byzuntina C. Koch), or with comparable preparations made from oats infected by the MAV strain of BYDV, a strain transmitted specifically by Macrosiphum avenue (Fabricius)(2, S). For both kinds of preparations, intramuscular injections of 1.0-3.3 ml of antigen emulsified in Freund’s incomplete adjuvant were made on days 1, 4, 8, and 10. In addition, those rabbits immunized with virus received intravenous injections of 0% 1.5 ml on days 2, 3, and 9. The antigen preparations used for immunization, made