Profiling of molecular interactions in real time using acoustic detection.
Profiling of molecular interactions in real time using acoustic detection.
复制标题
使用声学检测实时分析分子相互作用。
DOI：
10.1016/j.bios.2006.10.019
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发表时间：
2007
影响因子：
12.6
通讯作者：
Cooper,MatthewA
中科院分区：
文献类型：
--
作者：
Godber,Benjamin;Frogley,Mark;Rehak,Marian;Sleptsov,Alexander;Thompson,KevinSJ;Uludag,Yildiz;Cooper,MatthewA
利用共振石英晶体直接检测分析物与受体的结合的声传感器在临床相关分析物的定量中发现越来越多的实用性。我们开发了一种新的声学检测技术，我们称之为共振声学分析（RAP™）。该技术建立在“石英晶体微天平”或“QCM”的基础上，具有几个关键的附加功能，包括双通道或四通道自动样品输送，在线参考和预涂有易于使用的表面化学物质的微流体传感器“盒”。示例应用程序描述肌红蛋白浓度和其相互作用动力学的定量，并为酶辅因子特异性的排名。
Acoustic sensors that exploit resonating quartz crystals to directly detect the binding of an analyte to a receptor are finding increasing utility in the quantification of clinically relevant analytes. We have developed a novel acoustic detection technology, which we term resonant acoustic profiling (RAP™). This technology builds on the fundamental basics of the “quartz crystal microbalance” or “QCM” with several key additional features including two- or four-channel automated sample delivery, in-line referencing and microfluidic sensor ‘cassettes’ that are pre-coated with easy-to-use surface chemistries. Example applications are described for the quantification of myoglobin concentration and its interaction kinetics, and for the ranking of enzyme-cofactor specificities.