LYS3 encodes a prolamin-box-binding transcription factor that controls embryo growth in barley and wheat
LYS3 encodes a prolamin-box-binding transcription factor that controls embryo growth in barley and wheat
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DOI：
10.1101/2019.12.18.880955
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发表时间：
2019-12
影响因子：
3.8
通讯作者：
Beata Orman-Ligeza;P. Borrill;T. Chia;Marcella Chirico;J. Doležel;Sinéad Drea;Miroslava Karafiátová;Nicole Schatlowski;C. Solomon;B. Steuernagel;B. Wulff;C. Uauy;Kay Trafford
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作者：
Beata Orman-Ligeza;P. Borrill;T. Chia;Marcella Chirico;J. Doležel;Sinéad Drea;Miroslava Karafiátová;Nicole Schatlowski;C. Solomon;B. Steuernagel;B. Wulff;C. Uauy;Kay Trafford
大麦中LYS 3位点的突变对籽粒发育具有多种影响，包括胚大小的增加和胚乳淀粉含量的减少。通过遗传作图鉴定了LYS 3相关基因，并在所有四个大麦lys 3等位基因中鉴定了该基因的突变。LYS 3编码称为醇溶谷蛋白结合因子（PBF）的转录因子。利用小麦TILLING突变体证实了它在控制胚大小中的作用。为了了解PBF如何控制胚胎发育，我们研究了其在发育中的谷物中的时空表达模式。PBF基因在胚乳和胚中表达，但在这些器官中表达的时间不同。PBF在野生型胚胎中的表达先于lys 3突变体中胚胎增大的发生，表明PBF抑制胚胎生长。我们预测了PBF在小麦中的下游靶基因，发现它们参与了广泛的生物学过程，包括器官发育和淀粉代谢。我们的工作表明，PBF可能通过单独的基因控制网络影响胚大小和胚乳淀粉合成。亮点LYS 3编码一种称为醇溶蛋白结合因子（PBF）的转录因子，该因子仅在谷物中表达。小麦和大麦LYS 3/PBF突变体具有增大的胚，表明该基因抑制胚生长。小麦PBF基因的下游靶基因可能参与器官发育和淀粉代谢等多种生物学过程。
Mutations at the LYS3 locus in barley have multiple effects on grain development, including an increase in embryo size and a decrease in endosperm starch content. The gene underlying LYS3 was identified by genetic mapping and mutations in this gene were identified in all four barley lys3 alleles. LYS3 encodes a transcription factor called Prolamin Binding Factor (PBF). Its role in controlling embryo size was confirmed using wheat TILLING mutants. To understand how PBF controls embryo development, we studied its spatial and temporal patterns of expression in developing grains. The PBF gene is expressed in both the endosperm and the embryos, but the timing of expression in these organs differs. PBF expression in wild-type embryos precedes the onset of embryo enlargement in lys3 mutants, suggesting that PBF suppresses embryo growth. We predicted the down-stream target genes of PBF in wheat and found them to be involved in a wide range of biological processes, including organ development and starch metabolism. Our work suggests that PBF may influence embryo size and endosperm starch synthesis via separate gene control networks. HIGHLIGHTS LYS3 encodes a transcription factor called Prolamin Binding Factor (PBF) that is expressed in grains only. Wheat and barley LYS3/PBF mutants have enlarged embryos suggesting that this gene suppresses embryo growth. The down-stream target genes of PBF in wheat are predicted to be involved in a wide range of biological processes including organ development and starch metabolism.