ISOLATION OF ESCHERICHIA-COLI SEROTYPE O157-H7 AND OTHER SHIGA-LIKE-TOXIN-PRODUCING ESCHERICHIA-COLI FROM DAIRY-CATTLE
ISOLATION OF ESCHERICHIA-COLI SEROTYPE O157-H7 AND OTHER SHIGA-LIKE-TOXIN-PRODUCING ESCHERICHIA-COLI FROM DAIRY-CATTLE
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DOI：
10.1128/jcm.29.5.985-989.1991
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发表时间：
1991-05-01
影响因子：
9.4
通讯作者：
WACHSMUTH, IK
中科院分区：
文献类型：
--
作者：
WELLS, JG;SHIPMAN, LD;WACHSMUTH, IK
在调查两例与食用生牛奶有关的溶血性尿毒症综合征散发病例和一起由大肠杆菌血清型O157:H7引起的肠胃炎和溶血性尿毒症综合征暴发期间，我们检测了健康牛的1,266份粪便标本。我们在22个农场、一个畜牧场和一个包装车间收集了小母牛、小牛和成年奶牛的标本。我们还从一家餐馆收集了3个生汉堡样本，从两个农场收集了23个生牛奶样本。所有标本均采用山梨醇-麦康基琼脂、H固定化、O157凝集和组织培养细胞毒性检测大肠杆菌O157:H7。大肠杆菌O157:H7从22个农场的16头小母牛或小牛和1头成年奶牛、1头放养小牛、2份牛肉标本和1份原料奶样本中分离出来。通过检测菌落扫描的多粘菌素B提取物，然后检测单个菌落的毒素产生，还检查了所选粪便标本中是否存在其他志贺样产毒大肠杆菌（SLTEC）。除O157外，其他SLTEC从调查的10个养殖场中的8个养殖场和畜舍中分离出来；8%的成年奶牛和19%的小母牛和犊牛呈SLTEC阳性。有几只动物仅通过菌落扫描呈SLTEC阳性。这项调查表明，奶牛是大肠杆菌O157:H7和其他SLTEC的宿主。
We examined 1,266 fecal specimens from healthy cattle during the investigations of two sporadic cases of hemolytic uremic syndrome associated with raw milk consumption and an outbreak of gastroenteritis and hemolytic uremic syndrome caused by Escherichia coli serotype O157:H7. We collected specimens from heifers, calves, and adult cows on 22 farms, in a stockyard, and in a packing house. We also collected 3 raw hamburger specimens from a restaurant and 23 raw milk samples from two farms. All specimens were examined for E. coli O157:H7 by using sorbitol-MacConkey agar, H immobilization, O157 agglutination, and tissue culture cytotoxicity. E. coli O157:H7 was isolated from 16 heifers or calves and 1 adult cow on 22 farms, 1 stockyard calf, 2 beef specimens, and 1 raw milk sample. Selected fecal specimens were also examined for the presence of other Shiga-like-toxin-producing E. coli (SLTEC) by testing polymyxin B extracts of colony sweeps and then testing individual colonies for toxin production. SLTEC other than O157 was isolated from 8 of 10 farms investigated and from the stockyard; 8% of adult cows and 19% of heifers and calves were positive for SLTEC. Several animals were positive for SLTEC by colony sweep only. This investigation demonstrates that dairy cattle are a reservoir of E. coli O157:H7 and other SLTEC.