A Colorimetric CMOS-Based Platform for Rapid Total Serum Cholesterol Quantification
A Colorimetric CMOS-Based Platform for Rapid Total Serum Cholesterol Quantification
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DOI：
10.1109/jsen.2016.2629018
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发表时间：
2017-01-15
影响因子：
4.3
通讯作者：
Cumming, David R. S.
中科院分区：
文献类型：
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作者：
Al-Rawhani, Mohammed A.;Cheah, Boon Chong;Cumming, David R. S.
胆固醇水平升高与患心血管疾病和其他疾病的风险更大相关，使其成为一次性生物传感器检测的首选对象，用于快速护理点诊断。量化人类血清中胆固醇水平的方法之一是使用光学介导酶分析和台式分光光度计。这种设备的体积大，耗电大，限制了它在实验室的使用。在这里，我们提出了一种新的一次性传感平台，该平台基于互补金属氧化物半导体工艺，用于纯血清中总胆固醇的定量。我们实现的平台包括容易大规模制造的组件，这些组件利用胆固醇氧化酶和胆固醇酯酶反应的比色变化。我们已经证明，我们的定量结果与台式分光光度计所获得的结果相当。利用所实现的装置，我们测量了人血清中的胆固醇浓度，低至29微米，检测下限为13微米，比平均生理范围低约400倍，这意味着我们的装置也有潜力用于需要更高灵敏度的应用。
Elevated cholesterol levels are associated with a greater risk of developing cardiovascular disease and other illnesses, making it a prime candidate for detection on a disposable biosensor for rapid point of care diagnostics. One of the methods to quantify cholesterol levels in human blood serum uses an optically mediated enzyme assay and a bench top spectrophotometer. The bulkiness and power hungry nature of the equipment limits its usage to laboratories. Here, we present a new disposable sensing platform that is based on a complementary metal oxide semiconductor process for total cholesterol quantification in pure blood serum. The platform that we implemented comprises readily mass-manufacturable components that exploit the colorimetric changes of cholesterol oxidase and cholesterol esterase reactions. We have shown that our quantification results are comparable to that obtained by a bench top spectrophotometer. Using the implemented device, we have measured cholesterol concentration in human blood serum as low as 29 mu M with a limit of detection at 13 mu M, which is approximately 400 times lower than average physiological range, implying that our device also has the potential to be used for applications that require greater sensitivity.