Cellular Model of p21-Induced Senescence.
Cellular Model of p21-Induced Senescence.
复制标题
DOI：
10.1007/978-1-4939-6670-7_3
复制
发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Broude EV
中科院分区：
文献类型：
--
作者：
Shtutman M;Chang BD;Schools GP;Broude EV
细胞衰老是从胚胎发育到衰老的一个独特的正常生理过程，也因其与广泛的病理条件相关而闻名。因此，可靠的细胞衰老模型仍然是研究衰老相关变化和人类疾病不可或缺的工具。在这里，我们描述了一个模型的HT 1080纤维肉瘤细胞与诱导衰老表型。这些细胞配备有lac阻遏物和在lac阻遏物调节的启动子控制下的外源p21。衰老表型在这些细胞中通过异丙基-β-d-硫代半乳糖苷（IPTG）诱导的衰老相关细胞周期抑制剂p21 Wafl/Clp 1/Sdi 1的表达诱导。
Cellular senescence is a unique process of normal physiology, from embryonic development to aging, also known for its association with a broad range of pathological conditions. Therefore a reliable model of cellular senescence remains an indispensable tool for the investigation of senescence-associated changes and human disease. Here we describe a model of HT1080 fibrosarcoma cells with an inducible senescence phenotype. These cells are equipped with the lac repressor and exogenous p21 under the control of a lac repressor regulated promoter. The senescent phenotype is induced in these cells by isopropyl-β-d-thiogalactopyranoside (IPTG)-inducible expression of senescence-associated cell cycle inhibitor p21Wafl/Clp1/Sdi1.