Bacteriophage acquisition restores protective mutualism
Bacteriophage acquisition restores protective mutualism
复制标题
噬菌体的获得恢复了保护性互利共生
DOI：
10.1099/mic.0.000816
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发表时间：
2019
期刊：
影响因子：
1.5
通讯作者：
Oliver, Kerry M.
中科院分区：
文献类型：
--
作者：
Lynn-Bell, Nicole L.;Strand, Michael R.;Oliver, Kerry M.
昆虫经常被遗传的兼性共生体感染，已知这些共生体提供一系列条件有益的服务，包括宿主保护。豌豆蚜（Acyrthosiphon pisum）经常携带防御汉米尔顿氏菌（Hamiltonella defensa）及其相关噬菌体A。豌豆次生内共生体（APSE）赋予保护免受一个重要的天敌，寄生蜂Aphidius ervi。先前的研究表明，噬菌体APSE的自发丧失导致保护性表型的完全丧失。在这里，我们证明了APSE可以通过实验转移到无噬菌体（即非保护性）的Hamiltonellastrain。出乎意料的是，单独注射噬菌体颗粒的试验失败了，只有同时注射APSE和汉密尔顿杆菌时才能成功转移。转移后，APSE的稳定建立完全恢复了抗寄生虫的防御。因此，与可遗传的细菌共生体相关的微生物可以在共生体菌株之间水平移动，促进生态重要性状的快速转移，尽管自然屏障可能阻止定期交换。
Insects are frequently infected with inherited facultative symbionts known to provide a range of conditionally beneficial services, including host protection. Pea aphids (Acyrthosiphon pisum) often harbour the bacteriumHamiltonella defensa ,which together with its associated bacteriophageA. pisumsecondary endosymbiont (APSE) confer protection against an important natural enemy, the parasitic waspAphidius ervi. Previous studies showed that spontaneous loss of phage APSE resulted in the complete loss of the protective phenotype. Here, we demonstrate that APSEs can be experimentally transferred into phage-free (i.e. non-protecting)Hamiltonellastrains. Unexpectedly, trials using injections of phage particles alone failed, with successful transfer occurring only when APSE andHamiltonellawere simultaneously injected. After transfer, stable establishment of APSE fully restored anti-parasitoid defenses. Thus, phages associated with heritable bacterial symbionts can move horizontally among symbiont strains facilitating the rapid transfer of ecologically important traits although natural barriers may preclude regular exchange.