A SYSTEMATIC ASSESSMENT OF MORCHELLA USING RFLP ANALYSIS OF THE 28S RIBOSOMAL-RNA GENE
A SYSTEMATIC ASSESSMENT OF MORCHELLA USING RFLP ANALYSIS OF THE 28S RIBOSOMAL-RNA GENE
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DOI：
10.2307/3760589
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发表时间：
1994-11-01
期刊：
影响因子：
2.8
通讯作者：
ROYSE, DJ
中科院分区：
文献类型：
--
作者：
BUNYARD, BA;NICHOLSON, MS;ROYSE, DJ
羊肚菌(Morchella spp.)是北美野外发现的最珍贵的食用菌之一。由于环境条件可能会导致该属的成员在形态上发生变化，因此物种的数量一直存在争议。一些作者将该属划分为三到五个物种，而另一些作者则认为多达50个物种。从羊肚菌属及其亲缘关系较近的一属(羊肚菌属)中分离到DNA。用聚合酶链式反应扩增核糖体DNA重复序列的大亚基，然后用限制性内切酶进行酶切。在所调查的品系中发现了限制性片段长度多态性，并用于将这些品系划分为基因类群。同一物种的地理隔离种群之间的遗传变异可能比两个假定不同的物种之间的遗传变异更大。根据限制数据构建的系统发育树表明，黑羊鼠(M.angusticep、M.elata和M.conica)和黄色羊鼠(M.esculenta、M.crassipe和M.deliciosa)是独立的分类群。
Morels (Morchella spp.) are one of the most highly prized edible mushrooms found in the wild of North America. Because environmental conditions can cause members of the genus to vary morphologically, the number of species has been disputed. Some authors classify the genus into three to five species, while others argue for as many as 50 species. DNA from lines of Morchella and a closely related genus (Verpa) was isolated. The large subunit of the ribosomal DNA repeat was amplified using the polymerase chain reaction and then digested with restriction enzymes. Restriction fragment length polymorphisms were found among the lines investigated and used to separate the lines into genotypic classes. More genetic variation may occur between geographically isolated populations of the same species than between two putatively distinct species. The phylogenetic tree developed from restriction data suggests that the black morels (M. angusticeps, M. elata, and M. conica) and the yellow morels (M. esculenta, M. crassipes, and M. deliciosa) are separate taxonomic groups.