Expression of G protein-coupled receptor-30, a g protein-coupled membrane estrogen receptor, in oxytocin neurons of the rat paraventricular and supraoptic nuclei
Expression of G protein-coupled receptor-30, a g protein-coupled membrane estrogen receptor, in oxytocin neurons of the rat paraventricular and supraoptic nuclei
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DOI：
10.1210/en.2007-0436
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发表时间：
2007-12-01
期刊：
影响因子：
4.8
通讯作者：
Kawata, Mitsuhiro
中科院分区：
文献类型：
--
作者：
Sakamoto, Hirotaka;Matsuda, Ken-Ichi;Kawata, Mitsuhiro
雌激素对室旁核和视上核的大细胞催产素（OT）神经元的调节作用已有充分记录。尽管已经描述了核雌激素受体-β而非雌激素受体-α在OT神经元中的表达和分布，但核受体可能无法解释下丘脑OT神经元中雌激素功能的所有方面。最近，雌激素的 G 蛋白偶联受体 (GPR) GPR30 已被鉴定为几种癌细胞系中的膜定位雌激素受体。因此，在本研究中，我们研究了 GPR30 在大细胞 OT 神经元中的表达和定位，以了解这些神经元内雌激素快速作用的模式。在这里，我们发现，在室旁核和视上核中，GPR30 在大细胞 OT 神经元中以 mRNA 和蛋白质水平表达，但在加压素神经元中不表达。细胞内细胞器特异性标记物和免疫电镜显示GPR30主要定位于神经元的高尔基体，但在细胞表面无法检测到。此外，在神经垂体中也检测到GPR30的表达。这些结果表明，GPR30 可能主要作为下丘脑-神经垂体系统中雌激素作用的非基因组转导器。
The regulatory actions of estrogens on magnocellular oxytocin (OT) neurons of the paraventricular and supraoptic nuclei are well documented. Although the expression and distribution of nuclear estrogen receptor-beta, but not estrogen receptor-alpha, in the OT neuron has been described, the nuclear receptors may not explain all aspects of estrogen function in the hypothalamic OT neuron. Recently a G protein-coupled receptor (GPR) for estrogens, GPR30, has been identified as a membrane-localized estrogen receptor in several cancer cell lines. In this study, we therefore investigated the expression and localization of GPR30 in magnocellular OT neurons to understand the mode of rapid estrogen actions within these neurons. Here we show that, in the paraventricular nucleus and supraoptic nucleus, GPR30 is expressed in magnocellular OT neurons at both mRNA and protein levels but is not expressed in vasopressin neurons. Specific markers for intracellular organelles and immunoelectron microscopy revealed that GPR30 was localized mainly in the Golgi apparatus of the neurons but could not be detected at the cell surface. In addition, the expression of GPR30 is also detected in the neurohypophysis. These results suggest that GPR30 may serve primarily as a nongenomic transducer of estrogen actions in the hypothalamo-neurohypophyseal system.