Remodeling of the Streptococcus mutans proteome in response to LrgAB and external stresses.
Remodeling of the Streptococcus mutans proteome in response to LrgAB and external stresses.
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DOI：
10.1038/s41598-017-14324-w
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发表时间：
2017-10-25
影响因子：
4.6
通讯作者：
Rice KC
中科院分区：
文献类型：
--
作者：
Ahn SJ;Gu T;Koh J;Rice KC
变形链球菌Cid/Lrg系统代表了研究这种生物体如何承受口腔中遇到的各种压力的理想模型。lrgAB的突变使S.变形杆菌对氧化、热和万古霉素应激更敏感。在这里，我们进行了一个全面的蛋白质组学实验，使用无标记定量质谱比较野生型UA 159和lrgAB突变株在这些相同的压力下的蛋白质组变化。重要的是，许多鉴定的蛋白质显示出惊人的大倍数变化，或完全抑制或新诱导响应于特定的胁迫条件。值得注意的压力蛋白质组的变化发生在各种功能类别，包括氨基酸生物合成，能量代谢，蛋白质合成，运输/结合，转录/反应调节。在非应激生长条件下，lrgAB的突变显著改变了76种蛋白质的丰度（倍数变化>1.4，或<0.6，p值<0.05），并且其中几种与野生型菌株的应激蛋白质组相匹配。有趣的是，蛋白质组变化与相应的RNA-seq转录组学研究之间的统计相关性相对较低（rho（ρ）<0.16），表明适应新环境可能需要彻底的蛋白质组更新或代谢重塑。总的来说，这项研究加强了LrgAB的重要性，S。变形菌应激反应
The Streptococcus mutans Cid/Lrg system represents an ideal model to study how this organism withstands various stressors encountered in the oral cavity. Mutation of lrgAB renders S. mutans more sensitive to oxidative, heat, and vancomycin stresses. Here, we have performed a comprehensive proteomics experiment using label-free quantitative mass spectrometry to compare the proteome changes of wild type UA159 and lrgAB mutant strains in response to these same stresses. Importantly, many of identified proteins showed either a strikingly large fold-change, or were completely suppressed or newly induced in response to a particular stress condition. Notable stress proteome changes occurred in a variety of functional categories, including amino acid biosynthesis, energy metabolism, protein synthesis, transport/binding, and transcriptional/response regulators. In the non-stressed growth condition, mutation of lrgAB significantly altered the abundance of 76 proteins (a fold change >1.4, or <0.6, p-value <0.05) and several of these matched the stress proteome of the wild type strain. Interestingly, the statistical correlation between the proteome changes and corresponding RNA-seq transcriptomic studies was relatively low (rho(ρ) <0.16), suggesting that adaptation to a new environment may require radical proteome turnover or metabolic remodeling. Collectively, this study reinforces the importance of LrgAB to the S. mutans stress response.
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DOI：
10.1083/jcb.93.1.97
发表时间：
1982-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Fujiki Y;Hubbard AL;Fowler S;Lazarow PB
通讯作者：
Lazarow PB
影响因子：
3.7
作者：
Garcia SS;Du Q;Wu H
通讯作者：
Wu H
影响因子：
4.4
作者：
Bryan, Joshua D.;Liles, Roxanne;Shelver, Daniel
通讯作者：
Shelver, Daniel
影响因子：
3.2
作者：
Dufour, Delphine;Cordova, Martha;Levesque, Celine M.
通讯作者：
Levesque, Celine M.
影响因子：
4.4
作者：
Ahn, Sang-Joon;Rice, Kelly C.
通讯作者：
Rice, Kelly C.