Differential diagnosis between complete and partial mole by TSSC3 antibody completely correlates to DNA diagnosis
Differential diagnosis between complete and partial mole by TSSC3 antibody completely correlates to DNA diagnosis
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DOI：
10.1097/01.pas.0000162757.91649.a3
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发表时间：
2005-09-01
影响因子：
--
通讯作者：
Wake, N
中科院分区：
文献类型：
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作者：
Kato, H;Matsuda, T;Wake, N
完全性葡萄胎 (CHM) 是一种无卵雄激素受精。 CHM 病例表现出绒毛普遍肿胀，并且已知与持续性疾病或癌症高度相关。相比之下，部分葡萄胎（PHM）也表现出绒毛间特征性的水肿变化，但继发性疾病的发生率相对较低。因为 PHM 是由一个卵子和两个精子受精的，而 CHM 是由一个卵子和两个精子受精的。仅通过一两个精子，我们考虑了母体印记基因是否有助于实现鉴别诊断。通过实施微阵列技术来评估 CHM 中印记基因的有效性。在检查的基因中，TSSC3、SLC22A1L、KCNQ1 和核心蛋白聚糖被下调，并且 TSSC3 在这些基因中受到最明显的抑制。本研究对20例经DNA多态性确诊的CHM病例进行了调查。根据蛋白质印迹分析确定，在所有这些情况下，TSSC3 的表达完全不存在。相反，这里检查了 12 例 PHM，也是通过 DNA 多态性诊断的；在这12个病例中，TSSC3均被发现正常表达。免疫组织化学 (IHC) 分析也产生了相同的结果。 CHM 病例中 TSSC3 的完全沉默将为胎盘磨牙病变的诊断提供一种新颖、便捷的策略。
Complete hydatidiform moles (CHMs) are a type of androgenetic fertilization without an ovum. Cases of CHM exhibit a generalized swelling of the villi and are known to be highly associated with persistent, disease or carcinoma. In contrast, partial hydatidiform moles (PHMs) also show characteristic hydropic changes among the villi, but the incidence of secondary disease is relatively low. Because PHMs are fertilized by one ovum and two sperm and CHMs are fertilized. by one or two sperm alone, we considered whether or not maternally imprinted genes might be useful for achieving a differential diagnosis. The validity of the imprinted genes in CHMs was assessed by implementation of a microarray technique. Among the genes examined, TSSC3, SLC22A1L, KCNQ1, and Decorin were shown to be down-regulated, and TSSC3 was the most markedly suppressed of these genes. In this study, 20 cases of CHM, the diagnosis of which was confirmed by DNA polymorphism, were investigated. In all of these cases, the expression of TSSC3 was completely absent, as determined by Western blot analysis. Conversely, 12 cases of PHM, also diagnosed by DNA polymorphism, were examined here; in all of these 12 cases, TSSC3 Was found to be expressed normally. Immunohistochemical (IHC) analysis also produced the same results. The complete silencing of TSSC3 in cases of CHM will provide a novel, convenient strategy for the diagnosis of molar lesions in the placenta.