Bradyrhizobium valentinum sp nov., isolated from effective nodules of Lupinus mariae-josephae, a lupine endemic of basic-lime soils in Eastern Spain
Bradyrhizobium valentinum sp nov., isolated from effective nodules of Lupinus mariae-josephae, a lupine endemic of basic-lime soils in Eastern Spain
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DOI：
10.1016/j.syapm.2014.05.002
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发表时间：
2014-07-01
影响因子：
3.4
通讯作者：
Ruiz-Argueeso, Tomas
中科院分区：
文献类型：
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作者：
Duran, David;Rey, Luis;Ruiz-Argueeso, Tomas
从毛叶羽扇豆固氮根瘤中分离到的细菌菌株通过遗传、表型和共生的方法进行了鉴定。对16S rRNA基因的分析表明，它们与慢生根瘤菌USDA 76(T)、马铃薯根瘤菌PAC48(T)、吉卡米芽孢杆菌PAC68(T)、雷氏芽孢杆菌Ro19(T)和拉布拉比慢生根瘤菌CCBAU 23086(T)同源性超过99.0%。连锁看家基因recA、atpD和glnII的系统发育分析表明，该菌株是一个慢生根瘤菌新种，与B.lablabi CCBAU 23086(T)、B.jicamae PAC68(T)和‘B.retamae’Ro19(T)的同源性分别为92.1%、91.9%和90.8%。这些结果与使用相关菌株获得的基因组序列草案计算的平均核苷酸同一性(ANIM)计算的总体基因组同一性一致。虽然马立克氏乳杆菌LmjM3(T)/LmjM6菌株的anim值为99.2%，但它们之间的差异显著(
Bacterial strains isolated from nitrogen-fixing nodules of Lupinus mariae-josephae have been characterized following genetic, phenotypic and symbiotic approaches. Analysis of 16S rRNA genes placed them in a group together with Bradyrhizobium elkanii USDA 76(T), B. pachyrhizi PAC48(T), B. jicamae PAC68(T), 'B. retamae' Ro19(T) and B. lablabi CCBAU 23086(T) with over 99.0% identity. Phylogenetic analysis of concatenated housekeeping genes, recA, atpD and glnII, suggested that L. mariae-josephae strains represent a new Bradyrhizobium species, closely related to B. lablabi CCBAU 23086(T), B. jicamae PAC68(T) and 'B. retamae' Ro19(T) with 92.1, 91.9 and 90.8% identity, respectively. These results are consistent with overall genomic identities calculated as Average Nucleotide Identity (ANIm) using draft genomic sequences obtained for relevant strains. While L. mariae-josephae strains LmjM3(T)/LmjM6 exhibited a 99.2% ANIm value, they were significantly distant (