Sex reversal in the genus Oreochromis: Optimization of feminization protocol
Sex reversal in the genus Oreochromis: Optimization of feminization protocol
复制标题
DOI：
10.1111/j.1365-2109.1994.tb00696.x
复制
发表时间：
1994-01-01
期刊：
影响因子：
--
通讯作者：
Hulata, G.
中科院分区：
文献类型：
--
作者：
Rosenstein, S.;Hulata, G.
通过口服己烯雌酚(DES)和乙炔雌二醇(EES)对性腺未分化的莫氏罗非鱼(L.)和莫萨姆野生稻×欧洲野生稻为全雄杂交种。雌鱼在卵黄囊吸收后立即饲喂激素处理饲料，剂量分别为GT 10 mg/kg DES和GT 50 mg/kg EES。女性化的关键时期是一周左右。超过10天的处理不会导致更高的雌性比例，在卵黄囊吸收后一周开始的处理也没有效果。最优的衍生方案被应用于金鱼(Steindachner)，也导致了完全的女性化。
High rate of feminization was achieved by oral administration of diethylstilbestrol (DES) and ethynyloestradiol (EES) to gonadally undifferentiated Oreochromis mossambicus (L.) and O. mossambicus x O. urolepis hornorum Trewavas all-male hybrid. All-female groups were produced at doses gt 10 mg/kg DES and gt 50 mg/kg EES, when fry immediately after yolk-sac absorption were fed hormone-treated feed ad libitum. The critical period for feminization was about one week. Treatments longer than 10 days did not result in a higher proportion of females, and treatments starting later than one week after yolk-sac absorption were not effective. Optimal derived protocol was applied to O. aureus (Steindachner), resulting also in complete feminization.