POSSIBLE INFLUENCE OF TEMPORAL FACTORS IN ANDROGENIC RESPONSIVENESS OF UROGENITAL TISSUE RECOMBINANTS FROM WILD-TYPE AND ANDROGEN-INSENSITIVE (TFM) MICE
POSSIBLE INFLUENCE OF TEMPORAL FACTORS IN ANDROGENIC RESPONSIVENESS OF UROGENITAL TISSUE RECOMBINANTS FROM WILD-TYPE AND ANDROGEN-INSENSITIVE (TFM) MICE
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DOI：
10.1002/jez.1402050203
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发表时间：
1978-01-01
影响因子：
--
通讯作者：
LUNG, B
中科院分区：
文献类型：
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作者：
CUNHA, GR;LUNG, B
将来自野生型和雌性化（Tfm/Y）小鼠的胚胎和新生儿泌尿生殖道雏形的上皮和基质的组织重组体作为移植物在完整的雄性宿主中生长，并从形态学上分析雄激素反应。当胚胎野生型尿生殖窦（UGS）的间充质与来自胚胎野生型膀胱（B）的上皮结合时，上皮发育成类似前列腺的腺体结构。在相互重组体（B间充质+ UGS上皮）中，反应是混合的，一半的重组体表现出膀胱形态，一半表现出类膀胱形态。阴道样组织发生在雄激素不敏感的Tfm/Y间充质和野生型上皮的UGS重组体中，而前列腺形态在野生型间充质和Tfm/Y上皮的相互重组体中发展。这些观察结果表明，野生型间充质的存在下出现的雄激素依赖的形态发生在胚胎期的表达是必不可少的，Tfm/Y上皮细胞能够参与雄激素反应。相反，在用新生儿组织制备的类似重组体中，某些雄激素依赖性现象的表达可能不需要野生型泌尿生殖基质的存在，因为即使当该上皮与Tfm/Y泌尿生殖基质相关时，也会发生输精管上皮的高度和细胞分化的维持。泌尿生殖上皮对野生型（雄激素敏感）基质的需求可能随时间而变化。
Tissue recombinants of epithelium and stroma from embryonic and neonatal urogenital rudiments derived from wild-type and femininized (Tfm/Y) mice were grown as grafts in intact male hosts and analyzed morphologically for androgenic response. When mesenchyme of embryonic wild-type urogenital sinus (UGS) was associated with epithelium from embryonic wild-type bladder (B), the epithelium developed into glandular structures resembling prostate. In the reciprocal recombinant (B mesenchyme + UGS epithelium) the response was mixed, half of the recombinants exhibited bladder morphology and half exhibited prostatic-like morphology. Vaginal-like histogenesis occurred in UGS recombinants of androgen-insensitive Tfm/Y mesenchyme and wild-type epithelium, while prostatic morphology developed in reciprocal recombinants of wild-type mesenchyme and Tfm/Y epithelium. These observations demonstrate that the presence of wild-type mesenchyme appears essential for expression of androgen-dependent morphogenesis during embryonic periods; and that Tfm/Y epithelium is capable of participating in an androgenic response. Conversely, in similar recombinants prepared with neonatal tissues, the presence of wild-type urogenital stroma may not be required for expression of certain androgen-dependent phenomena since maintenance of the height and cytodifferentiation of ductus deferens epithelium occurs even when this epithelium is associated with Tfm/Y urogenital stroma. The requirement of urogenital epithelium for wild-type (androgen sensitive) stroma may vary temporally.