The effect of wild blueberry (Vaccinium angustifolium) consumption on postprandial serum antioxidant status in human subjects
The effect of wild blueberry (Vaccinium angustifolium) consumption on postprandial serum antioxidant status in human subjects
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DOI：
10.1079/bjn2002665
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发表时间：
2002-10-01
影响因子：
3.6
通讯作者：
Holub, BJ
中科院分区：
文献类型：
--
作者：
Kay, CD;Holub, BJ
本研究的目的是确定食用野生蓝莓（一种非营养性抗氧化植物化学物质的浓缩来源）是否会提高健康人餐后血清抗氧化水平。在一组8名中年男性受试者（38-54岁）中进行单盲交叉研究。受试者先食用高脂肪膳食和对照补充剂，一周后再食用同样的高脂肪膳食，并添加100.0 g冷冻干燥野生蓝莓粉。在肱静脉置管后，依次采集空腹和餐后血清样本，分析血脂、葡萄糖和血清抗氧化状态。采用氧自由基吸收能力（ORAC）法和总抗氧化状态（TAS）法测定血清抗氧化状态。野生蓝莓处理与ORAC试验（高氯酸（PCA）水溶性部分ORAC）确定的显著治疗效果相关，P=0.04)。血清抗氧化水平在1 h时显著高于对照组（ORAC(PCA)）（提高8.5%），P=0.02；TAS（增加4.5%），P=0.05)和4 h (ORAC（总）增加15.0%)，P=0.009；ORAC（丙酮）（高16.0%），P=0.007)。综上所述，食用野生蓝莓（一种具有高体外抗氧化性能的食物来源）与饮食诱导的体外血清抗氧化状态的增加有关。有研究表明，提高血清的抗氧化水平可能会降低许多慢性退行性疾病的风险。
The aim of the present study was to determine whether the consumption of wild blueberries (Vaccinium angustifolium), a concentrated source of non-nutritive antioxidant phytochemicals, would enhance postprandial serum antioxidant status in healthy human subjects. A single-blinded crossover study was performed in a group of eight middle-aged male subjects (38-54 years). Subjects consumed a high-fat meal and a control supplement followed 1 week later by the same high-fat meal supplemented with 100.0 g freeze-dried wild blueberry powder. Upon brachial vein catheterization, fasting and postprandial serum samples were taken sequentially and analysed for lipids and glucose and for serum antioxidant status. Serum antioxidant status was determined using the oxygen radical absorbance capacity (ORAC) assay and the total antioxidant status (TAS) assay. The wild-blueberry treatment was associated with a significant treatment effect as determined by the ORAC assay (water-soluble fraction ORAC(perchloric acid (PCA)), P=0.04). Significant increases in serum antioxidant status above the controls were observed at 1 h (ORAC(PCA) (8.5 % greater), P=0.02; TAS (4.5 % greater), P=0.05), and 4 h (ORAC(total) (15.0 % greater), P=0.009; ORAC(acetone) (16.0 % greater), P=0.007) post-consumption of the high-fat meal. In conclusion, the consumption of wild blueberries, a food source with high in vitro antioxidant properties, is associated with a diet-induced increase in ex vivo serum antioxidant status. It has been suggested that increasing the antioxidant status of serum may result in the reduced risk of many chronic degenerative diseases.