Investigating carbohydrate isomers by IMS-CID-IMS-MS: precursor and fragment ion cross-sections
Investigating carbohydrate isomers by IMS-CID-IMS-MS: precursor and fragment ion cross-sections
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DOI：
10.1039/c5an00840a
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发表时间：
2015-01-01
期刊：
影响因子：
4.2
通讯作者：
Clemmer, D. E.
中科院分区：
文献类型：
--
作者：
Gaye, M. M.;Kurulugama, R.;Clemmer, D. E.
离子迁移谱技术（IMS和IMS-IMS）结合碰撞诱导解离（CID）和质谱（MS）用于研究单锂化碳水化合物异构体的结构。除了一些有利的情况下，IMS-MS分析未衍生化的碳水化合物显示，大多数同量异位素前体离子具有相似的碰撞截面（CCS）。相反，通过IMS-CID-IMS-MS分析获得的同分异构碎片离子的ccs值通常是不同的，因此似乎可用作区分同分异构前体的手段。我们报告的前体和相关的碎片离子的CCS（在他）的值为三个单糖异构体（葡萄糖，半乳糖和果糖），十个二糖异构体（蔗糖，明串珠菌二糖，帕拉金糖，海藻糖，纤维二糖，β-龙胆二糖，异麦芽糖，麦芽糖，乳糖和蜜二糖），和三个三糖异构体（棉子糖，异麦芽三糖和麦芽三糖）。这些值被讨论作为区分前体碳水化合物的手段。
Ion mobility spectrometry techniques (IMS and IMS-IMS) combined with collision-induced dissociation (CID) and mass spectrometry (MS) are used to investigate the structures of singly-lithiated carbohydrate isomers. With the exception of some favorable cases, IMS-MS analyses of underivatized carbohydrates reveal that most isobaric precursor ions have similar collision cross sections (ccs). In contrast, ccs values for isomeric fragment ions obtained by IMS-CID-IMS-MS analysis are often different, and thus appear to be useful as a means of distinguishing the isomeric precursors. We report values of ccs (in He) for pre-cursor- and associated-fragment ions for three monosaccharide isomers (glucose, galactose and fructose), ten disaccharide isomers (sucrose, leucrose, palatinose, trehalose, cellobiose, beta-gentiobiose, isomaltose, maltose, lactose and melibiose), and three trisaccharide isomers (raffinose, melezitose and maltotriose). These values are discussed as a means of differentiating precursor carbohydrates.