Application of the combination index (CI)-isobologram equation to study the toxicological interactions of lipid regulators in two aquatic bioluminescent organisms
Application of the combination index (CI)-isobologram equation to study the toxicological interactions of lipid regulators in two aquatic bioluminescent organisms
复制标题
DOI：
10.1016/j.watres.2009.07.026
复制
发表时间：
2010-01-01
期刊：
影响因子：
12.8
通讯作者：
Fernandez-Pinas, Francisca
中科院分区：
文献类型：
--
作者：
Rodea-Palomares, Ismael;Petre, Alice L.;Fernandez-Pinas, Francisca
水环境中的药物并不是单独存在的，通常是以复杂的混合物形式存在的，它们的联合作用可能会对水生生物产生毒性。本文报道了一种广泛用于药理学研究药物相互作用的方法--组合指数(CI)-等温线方程在环境中的应用，以确定三种贝特类化合物对两种水生生物发光生物--费氏弧菌和自发发光蓝藻重组菌Anabaena CPB4337的毒理相互作用的性质。组合指数-等温线方程方法允许在弧菌试验中对协同作用、相加作用和拮抗作用进行计算机定量，在弧菌试验中，贝特组合在低作用水平表现出拮抗，在较高作用水平转变为相加效应或协同作用，相比之下，在鱼腥藻试验中，贝特组合在最低效应水平表现出很强的协同作用，在高效应水平表现出很强的拮抗作用。我们还在蓝藻试验中评估了三种贝特与真实废水样本的相互作用性质。我们建议组合指数-等值线方程方法可以作为生态毒理学评价的有用工具(C)2009爱思唯尔有限公司版权所有
Pharmaceuticals in the aquatic environment do not appear singly and usually occur as complex mixtures, whose combined effect may exhibit toxicity to the aquatic biota. We report an environmental application of the combination index (CI)-isobologram equation, a method widely used in pharmacology to study drug interactions, to determine the nature of toxicological interactions of three fibrates toward two aquatic bioluminescent organisms, Vibrio fischeri and the self-luminescent cyanobacterial recombinant strain Anabaena CPB4337 The combination index-isobologram equation method allows computerized quantitation of synergism, additive effect and antagonism In the Vibrio test, the fibrate combinations showed antagonism at low effect levels that turned into an additive effect or synergism at higher effect levels, by contrast, in the Anabaena test, the fibrate combinations showed a strong synergism at the lowest effect levels and a very strong antagonism at high effect levels We also evaluated the nature of the interactions of the three fibrates with a real wastewater sample in the cyanobacterial test We propose that the combination index-isobologram equation method can serve as a useful tool in ecotoxicological assessment (C) 2009 Elsevier Ltd All rights reserved