A non-cationic nucleic acid nanogel for the delivery of the CRISPR/Cas9 gene editing tool
A non-cationic nucleic acid nanogel for the delivery of the CRISPR/Cas9 gene editing tool
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用于传递 CRISPR/Cas9 基因编辑工具的非阳离子核酸纳米凝胶
DOI：
10.1039/c9nr05233j
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发表时间：
2019-10-07
期刊：
影响因子：
6.7
通讯作者：
Zhang, Chuan
中科院分区：
文献类型：
--
作者：
Ding, Fei;Huang, Xiangang;Zhang, Chuan
在本文中，我们报道了用于Cas9和单向导RNA（Cas9/sgRNA）复合物的细胞内递送的非阳离子DNA交联纳米凝胶。DNA接枝的聚己内酯刷（DNA-g-PCL）首先装载Cas9/sgRNA复合物，然后通过核酸杂交通过DNA接头交联以形成纳米级水凝胶，其中基因编辑工具嵌入并保护在内部。具有紧凑的结构，含有Cas9/sgRNA复合物的纳米凝胶表现出优异的抗核酸酶消化的生理稳定性和增强的细胞摄取效率，使递送系统成为靶基因组编辑的有前途的工具。
Herein, we report a non-cationic DNA-crosslinked nanogel for intracellular delivery of a Cas9 and single guide RNA (Cas9/sgRNA) complex. A DNA-grafted polycaprolactone brush (DNA-g-PCL) is first loaded with the Cas9/sgRNA complex and then crosslinked by DNA linkers via nucleic acid hybridization to form a nanosized hydrogel, in which the gene editing tools are embedded and protected inside. With compact architecture, the Cas9/sgRNA complex-containing nanogel exhibited excellent physiological stability against nuclease digestion and enhanced cellular uptake efficiency, making the delivery system a promising tool for target genome editing.