Research progress and application prospects of stable porcine pluripotent stem cells
Research progress and application prospects of stable porcine pluripotent stem cells
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DOI：
10.1093/biolre/ioac119
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发表时间：
2022-06
影响因子：
3.6
通讯作者：
Jinying Zhang;Minglei Zhi;Dengfeng Gao;Qianqian Zhu;Jie Gao;Gaoxiang Zhu;Suying Cao;Jianyong Han-J
中科院分区：
文献类型：
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作者：
Jinying Zhang;Minglei Zhi;Dengfeng Gao;Qianqian Zhu;Jie Gao;Gaoxiang Zhu;Suying Cao;Jianyong Han-J
多能干细胞（PSCs）具有无限的自我更新能力和多系分化潜能，在基础研究和生物医学科学中具有重要意义。以前曾报道在小鼠和人类中建立具有明确特征的PSCs，而稳定的大型动物PSCs的产生经历了相对较长的试验阶段，直到最近才取得突破。猪被认为是理想的动物模型，因为它们在生理和解剖学上与人类相似。猪PSCs的产生将为基础研究、基因工程、动物育种和养殖肉类提供细胞资源。本文综述了猪多能干细胞和重编程细胞的衍生研究进展，并阐述了猪胚胎发育过程中多能性变化的机制。这将有助于理解不同物种间参与胚胎发育的分化和保护，以及多能调控的信号通路。最后，我们还讨论了稳定猪psc的应用前景。尽管在猪干细胞领域仍存在挑战，但这些进展和观点将为今后的研究方向提供指导。
Abstract Pluripotent stem cells (PSCs) harbor the capacity of unlimited self-renewal and multilineage differentiation potential, which are crucial for basic research and biomedical science. Establishment of PSCs with defined features was previously reported from mice and humans, while generation of stable large animal PSCs has experienced a relatively long trial stage and only recently has made breakthroughs. Pigs are regarded as ideal animal models for their similarities in physiology and anatomy to humans. Generation of porcine PSCs would provide cell resources for basic research, genetic engineering, animal breeding, and cultured meat. In this review, we summarize the progress on the derivation of porcine PSCs and reprogramed cells and elucidate the mechanisms of pluripotency changes during pig embryo development. This will be beneficial for understanding the divergence and conservation between different species involved in embryo development and the pluripotent-regulated signaling pathways. Finally, we also discuss the promising future applications of stable porcine PSCs. Even though challenges remain in the field of porcine stem cells, these progress and viewpoints would provide guidance in future research direction.