The Contribution of Carbon and Water in Modulating Wood Formation in Black Spruce Saplings
The Contribution of Carbon and Water in Modulating Wood Formation in Black Spruce Saplings
复制标题
碳和水在调节黑云杉幼苗木材形成中的贡献
DOI：
10.1104/pp.15.01525
复制
发表时间：
2016-04-01
期刊：
影响因子：
7.4
通讯作者：
Rossi, Sergio
中科院分区：
文献类型：
--
作者：
Deslauriers, Annie;Huang, Jian-Guo;Rossi, Sergio
非结构性碳水化合物（NSC）在木质部的形成中起着至关重要的作用，并且与水一起代表植物生长的主要限制。我们评估了木质部发生和神经干细胞之间的关系，以（1）验证神经干细胞解释的方差和（2）确定内在（组织供应碳）和外在（水的可用性和温度）因素的影响。在2年期间，监测木材形成的黑云杉（Picea mariana）的树苗进行约1个月的干燥期在6月，暴露于不同的温度处理在温室中。与此同时，NSC浓度的测定是通过从两种组织（形成层和内部木质部）中提取糖化合物来进行的，这两种组织都可能为木材形成提供碳。混合效应模型用于评估和量化潜在的关系。总木质部细胞，说明分生组织的活动，被建模为水，蔗糖和D-松醇（条件r（2）为0.79）的函数。水分有效性是解释木质部细胞总产量的最重要因素，而碳的贡献较小。形成层在水分亏缺下停止分裂，可能是为了限制在没有足够水量的情况下仍处于分化状态的细胞数量。相比之下，碳因子在解释活细胞的变化方面被列为最重要的因素（条件r（2）为0.49），突出了木质部发育过程中的功能需求，其次是供应神经干细胞（形成层）和水的组织。这项研究准确地证明了碳和水在结构生长中的作用，表现为分生组织活动和组织形成。
Nonstructural carbohydrates (NSCs) play a crucial role in xylem formation and represent, with water, the main constraint to plant growth. We assessed the relationships between xylogenesis and NSCs in order to (1) verify the variance explained by NSCs and (2) determine the influence of intrinsic (tissue supplying carbon) and extrinsic (water availability and temperature) factors. During 2 years, wood formation was monitored in saplings of black spruce (Picea mariana) subjected to a dry period of about 1 month in June and exposed to different temperature treatments in a greenhouse. In parallel, NSC concentrations were determined by extracting the sugar compounds from two tissues (cambium and inner xylem), both potentially supplying carbon for wood formation. A mixed-effect model was used to assess and quantify the potential relationships. Total xylem cells, illustrating meristematic activity, were modeled as a function of water, sucrose, and D-pinitol (conditional r(2) of 0.79). Water availability was ranked as the most important factor explaining total xylem cell production, while the contribution of carbon was lower. Cambium stopped dividing under water deficit, probably to limit the number of cells remaining in differentiation without an adequate amount of water. By contrast, carbon factors were ranked as most important in explaining the variation in living cells (conditional r(2) of 0.49), highlighting the functional needs during xylem development, followed by the tissue supplying the NSCs (cambium) and water availability. This study precisely demonstrates the role of carbon and water in structural growth expressed as meristematic activity and tissue formation.