Integration of multiple signal transducing pathways on Fgf response elements of the Xenopus caudal homologue Xcad3
Integration of multiple signal transducing pathways on Fgf response elements of the Xenopus caudal homologue Xcad3
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DOI：
10.1242/dev.00718
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发表时间：
2003-10-01
期刊：
影响因子：
4.6
通讯作者：
Okamoto, H
中科院分区：
文献类型：
--
作者：
Haremaki, T;Tanaka, Y;Okamoto, H
沿着前后（AP）轴的早期神经模式似乎涉及许多信号转导通路，但这些通路中的每一个对于AP模式的确切作用以及它们如何与支配神经诱导步骤的信号整合还没有很好地理解。我们调查的性质成纤维细胞生长因子反应元件（FRE）在后神经基因，Xcad 3（非洲爪蟾尾同源），后神经发育起着至关重要的作用。我们提供的证据表明，FREs的Xcad 3广泛分布在其内含子序列，这些多个FREs包括Ets结合和Tcf/Lef-binding基序，并置。功能和物理分析表明，Fgf，Bmp和Wnt信号通路整合在这些FREs上，通过正作用的Ets和Sox家族转录因子和负作用的Tcf家族转录因子来调节Xcad 3在后神经管中的表达。
Early neural patterning along the anteroposterior (AP) axis appears to involve a number of signal transducing pathways, but the precise role of each of these pathways for AP patterning and how they are integrated with signals that govern neural induction step is not well understood. We investigate the nature of Fgf response element (FRE) in a posterior neural gene, Xcad3 (Xenopus caudal homologue) that plays a crucial role of posterior neural development. We provide evidence that FREs of Xcad3 are widely dispersed in its intronic sequence and that these multiple FREs comprise Ets-binding and Tcf/Lef-binding motifs that lie in juxtaposition. Functional and physical analyses indicate that signaling pathways of Fgf, Bmp and Wnt are integrated on these FREs to regulate the expression of Xcad3 in the posterior neural tube through positively acting Ets and Sox family transcription factors and negatively acting Tcf family transcription factor(s).