Genetic quality assurance and genetic monitoring of laboratory mice and rats: FELASA Working Group Report
Genetic quality assurance and genetic monitoring of laboratory mice and rats: FELASA Working Group Report
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DOI：
10.1177/0023677219867719
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发表时间：
2019-08-20
影响因子：
2.4
通讯作者：
Wedekind, Dirk
中科院分区：
文献类型：
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作者：
Benavides, Fernando;Ruelicke, Thomas;Wedekind, Dirk
遗传质量保证(QA)，包括近交系的遗传监测(GEMO)和遗传改变(GA)动物模型的背景特征(BC)，应该是实验室动物设施中任何QA计划的基本组成部分。遗传质量控制对于确保动物模型的有效性与健康和微生物学监测一样重要。应该要求使用实验室啮齿动物，主要是小鼠和大鼠的研究，利用基因定义的动物。这份由FELASA实验室小鼠遗传质量保证和遗传监测工作组提出的文件，描述了啮齿动物设施中遗传质量保证计划的目标和可用的方法。任何遗传QA计划的主要目标是：(A)核实近交系和亚种系的真实性和一致性，从而确保遗传上可靠的菌落维持；(B)检测可能的遗传污染；以及(C)准确描述遗传系的遗传组成。虽然本出版物主要关注小鼠和大鼠的遗传QA，但这些原理将适用于其他啮齿动物物种，其中一些在近交系和近交系种群的背景下被简要提及。
Genetic quality assurance (QA), including genetic monitoring (GeMo) of inbred strains and background characterization (BC) of genetically altered (GA) animal models, should be an essential component of any QA programme in laboratory animal facilities. Genetic quality control is as important for ensuring the validity of the animal model as health and microbiology monitoring are. It should be required that studies using laboratory rodents, mainly mice and rats, utilize genetically defined animals. This paper, presented by the FELASA Working Group on Genetic Quality Assurance and Genetic Monitoring of Laboratory Murines, describes the objectives of and available methods for genetic QA programmes in rodent facilities. The main goals of any genetic QA programme are: (a) to verify the authenticity and uniformity of inbred stains and substrains, thus ensuring a genetically reliable colony maintenance; (b) to detect possible genetic contamination; and (c) to precisely describe the genetic composition of GA lines. While this publication focuses mainly on mouse and rat genetic QA, the principles will apply to other rodent species some of which are briefly mentioned within the context of inbred and outbred stocks.