Neutrophil adhesion molecules in chronic hemodialysis patients.
Neutrophil adhesion molecules in chronic hemodialysis patients.
复制标题
慢性血液透析患者的中性粒细胞粘附分子。
作者：
P. Zachée;R. Daelemans;P. Pollaris;M. Boogaerts;R. Lins
采用流式细胞术检测10例慢性血液透析患者（CHD）透析前和透析中中性粒细胞（PMN）表面粘附分子LFA-1（CD 11 a-CD 18）、Mac-1（CD 11b-CD 18）和LAM-1的表达，并与年龄匹配的正常对照组进行比较。所有患者均使用聚丙烯腈或聚砜膜进行透析。为了了解透析间期的影响，我们比较了周一（3天未透析）和周三（2天未透析）的样本。未接受重组人促红细胞生成素（rHuEPO）治疗的患者组也被纳入，以分析r-HuEPO的影响。我们发现，在透析前和透析中的样本中，CHD患者和正常对照组中的CD 11 a的表达没有差异。血液透析与15分钟和30分钟样本中LAM-1的快速和显著降低相关（p < 0.05）。这种降低只是暂时的，在透析120分钟后恢复到接近透析前的水平。MAC-1在透析30分钟后显著增加（p < 0.01），并且在透析过程结束时仍保持在透析前水平之上（p < 0.01）。另一方面，我们发现在透析前样品中MAC-1显著上调（p < 0.005），LAM-1下调。我们进一步注意到在这些透析前样品中LAM-1的表达显著较低（82 +/-9.6%）（p < 0.005）。这些结果表明，“高MAC-1，低LAM-1”的中性粒细胞不仅是透析相关的现象，但他们已经存在之前的血液透析会议，也没有任何差异之间的透析时间或相比，r-HuEPO治疗。
The expression of the adhesion molecules LFA-1 (CD11a-CD18), Mac-1 (CD11b-CD18), and LAM-1 on predialysis and intradialysis neutrophils (PMN) was analyzed by flow cytometry in 10 chronic hemodialysis patients (CHD) and compared with age-matched normal controls. All patients were dialyzed either with a polyacrylonitrile, or a polysulfone membrane. To appreciate the influence of the interdialytic time, we compared the samples of Monday (3 days without dialysis), with those of Wednesday (2 days without dialysis). A patient group not treated with recombinant human erythropoietin (rHuEPO) was also included to analyze the influence of r-HuEPO. We found on the predialysis and intradialysis samples that the expression of CD11a was not different in the CHD patients and in the normal controls. Hemodialysis was associated with a rapid and significant reduction in LAM-1 on the 15- and 30-min samples (p < 0.05). This reduction was only transient, and returned to near predialysis levels after 120 min dialysis. The MAC-1 increased significantly after 30 min dialysis (p < 0.01), and remained at the end of the dialysis procedure still substantially above the predialysis levels (p < 0.01). On the other hand, we have found a significant (p < 0.005) up-regulating of the MAC-1, and a down-regulating of the LAM-1 in the predialysis samples. We noticed further a significantly lower expression (82 +/- 9.6%) for LAM-1 in these predialysis samples (p < 0.005). These results demonstrate that 'high MAC-1, low LAM-1' neutrophils were not only a dialysis-related phenomenon, but that they were already present before the hemodialysis session, nor was there any difference between the interdialytic times or compared with the r-HuEPO treatment.