Larval cuticular morphogenesis in the tobacco hornworm, Manduca sexta, and its hormonal regulation.
Larval cuticular morphogenesis in the tobacco hornworm, Manduca sexta, and its hormonal regulation.
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烟草天蛾幼虫角质层形态发生及其激素调节。
DOI：
10.1016/0012-1606(86)90166-1
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发表时间：
1986
影响因子：
2.7
通讯作者：
Riddiford,LM
中科院分区：
文献类型：
--
作者：
Wolfgang,WJ;Riddiford,LM
烟草角质层蛋白的合成和沉积在烟草角质层蛋白的合成和沉积过程中依次发生，并与角质层结构的变化有关。在摄食的最后一天（第3天），在变态开始前，许多囊内蛋白不再合成，新的27,000-Da多肽等电变体沉积在角质层中，形成比先前薄5- 10倍的薄片。在第1天使用一种类似于甲基戊二烯的幼年激素，通过防止第2天观察到的蜕皮激素滴度的上升，阻止了蛋白质合成和片层结构的这种变化。在无幼激素的情况下，25 ~ 100 ng/ml 20-羟基蜕皮激素可在体外诱导这些变化。因此，片层组装过程中的蜕皮变化似乎是由激素诱导的角质层蛋白质合成的变化引起的。
The sequential synthesis and deposition of larval cuticular proteins was followed during the final larval molt and the final larval instar of the tobacco hornwormManduca sextaand correlated with changes in cuticular structure. On the final day of feeding (Day 3) before the onset of metamorphosis many endocuticular proteins were no longer synthesized and new isoelectric variants of 27,000-Da polypeptides were deposited into the cuticle coincident with the formation of lamellae 5- to 10-fold thinner than those previously deposited. Application of a juvenile hormone analog methoprene on Day 1 prevented this change in protein synthesis and in lamellar structure by preventing the observed rise in the intermolt ecdysteroid titer on Day 2. These changes could be inducedin vitroby 25–100 ng/ml 20-hydroxyecdysone in the absence of juvenile hormone. Thus, the intermolt change in the lamellar assembly process appears to result from hormone-induced changes in cuticular protein synthesis.