Cytokeratin filaments are present in golden hamster oocytes and early embryos.
Cytokeratin filaments are present in golden hamster oocytes and early embryos.
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细胞角蛋白丝存在于金仓鼠卵母细胞和早期胚胎中。
DOI：
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
José F. David
中科院分区：
文献类型：
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作者：
C. Plancha;Maria Carmo;José F. David
应用光镜和电镜技术研究了金黄地鼠近期排卵卵母细胞、受精卵和早期胚胎中细胞角蛋白的表达。两种细胞角蛋白多肽（Mr 51，000和58，000）在卵母细胞裂解液中检测到免疫印迹使用多克隆抗血清前角蛋白。在卵母细胞中，细胞角蛋白呈片状聚集体，由10 nm的短纤维组成，形成分布在细胞质中的紧密网状结构。受精后，聚集体似乎合并，变得更大，并集中在皮层区域。观察到显著的免疫荧光原纤维将聚集体相互连接。在2-，4-和8-细胞胚胎中，细胞角蛋白丝的网络延伸到整个皮质和核周区域，而聚集体逐渐消失。
Light and electron microscope methods were used to study cytokeratin expression in the recently ovulated oocytes, fertilized eggs and early embryos from the golden hamster. Two cytokeratin polypeptides (Mr 51,000 and 58,000) were detected in oocyte lysates by immunoblotting using a polyclonal antiserum to prekeratin. In the oocyte, cytokeratin occurred as patchy aggregates consisting of short anastomosing 10-nm filaments that formed tight meshworks distributed throughout the cytoplasm. After fertilization the aggregates appeared to merge, becoming larger and concentrated at the cortical region. Prominent immunofluorescent fibrils were seen interconnecting the aggregates. In the 2-, 4- and 8-cell embryos, networks of cytokeratin filaments extended throughout the cortical and perinuclear regions, while aggregates progressively disappeared.