DEVELOPMENTAL EXPRESSION OF CRYSTALLIN GENES - INSITU HYBRIDIZATION REVEALS A DIFFERENTIAL LOCALIZATION OF SPECIFIC MESSENGER-RNAS
DEVELOPMENTAL EXPRESSION OF CRYSTALLIN GENES - INSITU HYBRIDIZATION REVEALS A DIFFERENTIAL LOCALIZATION OF SPECIFIC MESSENGER-RNAS
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DOI：
10.1016/0012-1606(87)90392-7
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发表时间：
1987-10-01
影响因子：
2.7
通讯作者：
SCHOENMAKERS, JGG
中科院分区：
文献类型：
--
作者：
VANLEEN, RW;BREUER, ML;SCHOENMAKERS, JGG
本文用原位杂交技术研究了αA-、β B_1-和γ-晶状体蛋白RNA在发育中的大鼠透镜中积累的时间和地点。αA-和γ-晶状体蛋白RNA首先在透镜囊泡中被检测到，而βB1-晶状体蛋白RNA则在初生纤维细胞伸长后才能被检测到。βB1-和γ-晶体蛋白RNA仅见于胎儿晶状体纤维细胞，α A-晶体蛋白mRNA也见于上皮细胞。在新生大鼠透镜分化区获得的杂交模式的定量表明，α A-晶状体蛋白RNA集中在皮质区。α B-晶体蛋白mRNA的分布模式与此一致。在胎鼠和新生鼠晶状体中，βB1-晶状体蛋白RNA的原位杂交检测相对较差。用该探针获得的颗粒密度从透镜的周边向内部增加，表明βB1-晶状体蛋白RNA在次生纤维细胞分化期间积累。γ-晶状体蛋白mRNA也获得了类似的积累模式，但出乎意料的是，这种RNA也可以在伸长的上皮细胞中检测到。我们的研究结果表明，γ-晶状体蛋白RNA开始积累，一旦可见的上皮细胞的伸长发生，在初级以及次级纤维细胞的分化。
The time and place of the accumulation of αA-, βB1- and γ-crystallin RNA in the developing rat lens have been studied byin situhybridization. αA- and γ-crystallin RNA were first detected in the lens vesicle, while βB1-crystallin RNA could be seen only after elongation of the primary fiber cells. Both βB1- and γ-crystallin RNA were confined to the fiber cells of fetal lenses, while αA-crystallin mRNA could also be detected in the epithelial cells. A quantification of the hybridization pattern obtained in the differentiation zone of the newborn rat lens showed that αA-crystallin RNA is concentrated in the cortical zone. αB-crystallin mRNA has the same distribution pattern. βB1-crystallin RNA was relatively poorly detectable byin situhybridization in both fetal and newborn rat lenses. The grain densities obtained with this probe increased from the periphery of the lens toward the interior, indicating that βB1-crystallin RNA accumulated during differentiation of the secondary fiber cells. A similar accumulation pattern was obtained for γ-crystallin mRNA, but, unexpectedly, this RNA could also be detected in the elongating epithelial cells. Our results show that γ-crystallin RNA starts to accumulate as soon as visible elongation of epithelial cells occurs, during differentiation of the primary as well as the secondary fiber cells.