A natural tandem array alleviates epigenetic repression of IPA1 and leads to superior yielding rice.
A natural tandem array alleviates epigenetic repression of IPA1 and leads to superior yielding rice.
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天然串联阵列减轻了 IPA1 的表观遗传抑制，从而培育出高产的水稻。
DOI：
10.1038/ncomms14789
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发表时间：
2017-03-20
影响因子：
16.6
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Zhang L;Yu H;Ma B;Liu G;Wang J;Wang J;Gao R;Li J;Liu J;Xu J;Zhang Y;Li Q;Huang X;Xu J;Li J;Qian Q;Han B;He Z;Li J
具有理想植株结构的超级杂交水稻品种在加强全球粮食安全方面至关重要。然而，其提高产量的分子机制仍不清楚。本研究在超级稻甬优12号（YY 12）及其相关品种中定位了一个QTL qWS 8/ipa 1 -2D。对qWS 8/ipa 1 -2D的深入遗传分子特征分析表明，这一新发现的QTL来自IPA 1上游的三个远端天然存在的串联重复序列，IPA 1是一个先前显示塑造水稻理想植株结构并大大提高籽粒产量的关键基因/位点。qWS 8/ipa 1 -2D基因座与IPA 1启动子处DNA甲基化降低和染色质更开放的状态相关，从而减轻了由附近异染色质介导的IPA 1的表观遗传抑制。我们的研究结果表明，IPA性状可以通过操纵IPA 1的表达进行微调，最佳的IPA 1表达/剂量可能会导致理想的产量，展示了一种实用的方法来有效地设计精英超级稻品种。培育具有理想植株结构的水稻提高了全世界的水稻产量。Zhang等人在这里表明，在超级稻品种YY 12中，这种理想的植物结构性状来自三个远端串联重复序列，这三个远端串联重复序列与开放的染色质状态和IPA 1基因表达的增加有关。
Super hybrid rice varieties with ideal plant architecture (IPA) have been critical in enhancing food security worldwide. However, the molecular mechanisms underlying their improved yield remain unclear. Here, we report the identification of a QTL, qWS8/ipa1-2D, in the super rice Yongyou12 (YY12) and related varieties. In-depth genetic molecular characterization of qWS8/ipa1-2D reveals that this newly identified QTL results from three distal naturally occurring tandem repeats upstream of IPA1, a key gene/locus previously shown to shape rice ideal plant architecture and greatly enhance grain yield. The qWS8/ipa1-2D locus is associated with reduced DNA methylation and a more open chromatin state at the IPA1 promoter, thus alleviating the epigenetic repression of IPA1 mediated by nearby heterochromatin. Our findings reveal that IPA traits can be fine-tuned by manipulating IPA1 expression and that an optimal IPA1 expression/dose may lead to an ideal yield, demonstrating a practical approach to efficiently design elite super rice varieties. Breeding of rice with ideal plant architecture has increased rice yield worldwide. Here Zhang et al. show that, in the super rice variety YY12, this ideal plant architecture trait arose from three distal tandem repeats that are associated with an open chromatin state and increased expression of the IPA1 gene.