Trypsin digestion in immunoperoxidase staining.

Trypsin digestion in immunoperoxidase staining.
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免疫过氧化物酶染色中的胰蛋白酶消化。

DOI:
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发表时间:
1980
影响因子:
3.2
通讯作者:
W. Mccaughey
W. Mccaughey
中科院分区:
生物学3区
文献类型:
--
作者:
N. Hautzer;J. F. Wittkuhn;W. Mccaughey

文献摘要

被引文献

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研究了胰蛋白酶消化对正常和异常组织石蜡切片的免疫过氧化物酶-PAP 染色的影响。胰蛋白酶处理显着减少了背景染色,但增强了特异性染色。因此,结果更容易阅读。讨论了这一结果的可能原因。
The effect of trypsin digestion on the immunoperoxidase-PAP staining of paraffin sections from normal and abnormal tissue was studied. Trypsin treatment reduced background staining considerably, but enhanced specific staining. Results were, therefore, much easier to read. Possible reasons for this result are discussed.