IDENTIFICATION OF HERPES-SIMPLEX VIRUS-DNA SEQUENCES WHICH ENCODE A TRANS-ACTING POLYPEPTIDE RESPONSIBLE FOR STIMULATION OF IMMEDIATE EARLY TRANSCRIPTION
IDENTIFICATION OF HERPES-SIMPLEX VIRUS-DNA SEQUENCES WHICH ENCODE A TRANS-ACTING POLYPEPTIDE RESPONSIBLE FOR STIMULATION OF IMMEDIATE EARLY TRANSCRIPTION
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DOI：
10.1016/0022-2836(84)90427-3
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发表时间：
1984-01-01
影响因子：
5.6
通讯作者：
PRESTON, CM
中科院分区：
文献类型：
--
作者：
CAMPBELL, MEM;PALFREYMAN, JW;PRESTON, CM
Herpes simplex virus (HSV) immediate early (IE) transcription is known to be stimulated by a structural component of the virion which interacts, either directly or indirectly, with specific regulatory sequences located far upstream from IE mRNA 5''-termini. The mapping and identification of the virion component was described. Cloned HSV DNA fragments derived from various parts of the genome were cotransfected into BHK cells together with chimeric plasmids which contained the thymidine kinase gene under IE control. Stimulation of thymidine kinase synthesis was elicited by cloned EcoRIi (0.63-0.72 map units). BamHIf (0.64-0.69) or EcoRIb (0.72-0.87). Cloned BamHIf had the same specificity as the virion component, since it stimulated thymidine kinase expression only from chimeric plasmids which contained functional IE-specific regulatory sequences. The effect of EcoRIb was not confined to plasmids with IE-specific regulatory regions, suggesting a more general stimulatory role for > 1 of the polypeptides encoded by this fragment. A subclone containing a 2.7 .times. 103 base-pair fragment of BamHIf(pMCl) was active in the cotransfection assay, and the effect was abolished by an 8 base-pair insertion into the middle of this fragment. The only polypeptide known to map entirely within the HSV genome region defined by pMCl was identified as the major tegument species Vmw65. The results therefore suggest that Vmw65 is the virion component which trans-activates HSV IE transcription.
