Membrane Permeability and the Loss of Germination Factor from Neurospora crassa at Low Water Activities
Membrane Permeability and the Loss of Germination Factor from Neurospora crassa at Low Water Activities
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DOI：
10.1128/jb.117.1.261-264.1974
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发表时间：
1974-01
影响因子：
3.2
通讯作者：
G. Charlang;N. Horowitz
中科院分区：
文献类型：
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作者：
G. Charlang;N. Horowitz
粗毛脉孢菌分生孢子在低水分活度(Aw)的缓冲液中孵育后，无论是使用电解液还是非电解液来降低aw值，都会释放出萌发所必需的成分以及260 nm的吸收物质和金刚三酮阳性物质。众所周知，氯仿和抗生素可以增加细胞膜的通透性，具有类似的效果。这表明，膜损伤发生在低Aw的介质中，通透性的增加是导致细胞成分释放的原因。在低Aw的培养基中造成的伤害大多是非致死的，当分生孢子转移到营养培养基上时，分生孢子会恢复。
Neurospora crassa conidia incubating in buffer at low water activities (aw) release a germination-essential component as well as 260-nm absorbing and ninhydrin-positive materials, regardless of whether an electrolyte or non-electrolyte is used to reduce aw. Chloroform and antibiotics known to increase cell-membrane permeability have a similar effect. This suggests that membrane damage occurs in media of low aw and that an increase in permeability is responsible for the release of cellular components. The damage caused in media of low aw is nonlethal in most cases, and the conidia recover when transferred to nutrient medium.