MOLECULAR ANALYSIS OF DS CONTROLLING ELEMENT MUTATIONS AT THE ADH1 LOCUS OF MAIZE
MOLECULAR ANALYSIS OF DS CONTROLLING ELEMENT MUTATIONS AT THE ADH1 LOCUS OF MAIZE
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DOI：
10.1126/science.223.4642.1265
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发表时间：
1984-01-01
期刊：
影响因子：
56.9
通讯作者：
PEACOCK, WJ
中科院分区：
文献类型：
--
作者：
SUTTON, WD;GERLACH, WL;PEACOCK, WJ
已分离到一个活跃的玉米Adh1-F基因，即ADS诱导的该基因的突变体，以及两个由Ac诱导的独立突变等位基因。Dsmutant与祖先等位基因的不同之处在于，它有一个405碱基对的插入，侧翼有一个8bp的直接重复。该重复是存在于该基因5‘非翻译区插入点的8个碱基对的重复。插入序列是富含AT的(A，腺嘌呤；T，胸腺嘧啶)，在其末端有11个碱基的反向重复序列。在回复突变体中，带有反向重复序列的插入片段被删除，但8个碱基对的直接重复序列保持修改形式。这些结果证实了405bp的序列是缺失的。该突变株中Adh1信使RNA水平较低，似乎使用了新的转录起始或RNA加工位点，或两者兼而有之。玉米基因组中至少有30个与该基因相关的序列。
An active maizeAdh1-Fgene, aDs-induced mutant of this gene, and two independentAc-induced revertant alleles have been isolated. TheDsmutant differs from the progenitor allele in having a 405-base pair insertion flanked by a direct repeat of 8 bp. The repeat is a duplication of the 8 bp existing at the point of insertion in the 5′ untranslated region of the gene. The insertion sequence is AT-rich (A, adenine; T, thymine) and has 11-bp inverted repeat sequences at its termini. In the revertants the insertion with its inverted repeats is deleted, but the 8-bp direct repeats remain in modified form. These results establish that the 405-bp sequence is aDselement. TheAdh1messenger RNA level is low in theDsmutant, and it appears that new sites for transcription initiation or RNA processing or both are used. There are at least 30 sequences in the maize genome related to theDselement.