Platinum(II)-Based Optical Probes for Imaging Quadruplex DNA Structures via Phosphorescence Lifetime Imaging Microscopy
Platinum(II)-Based Optical Probes for Imaging Quadruplex DNA Structures via Phosphorescence Lifetime Imaging Microscopy
复制标题
基于铂 (II) 的光学探针通过磷光寿命成像显微镜对四链体 DNA 结构进行成像
DOI：
10.1002/ange.202310402
复制
发表时间：
2023
影响因子：
--
通讯作者：
Berrones Reyes J
中科院分区：
文献类型：
--
作者：
Berrones Reyes J
G -四重体DNA是一种非规范结构，形成于基因组中富含鸟嘌呤的区域。越来越多的证据表明G -四重复合物具有重要的生物学功能，因此可视化这些结构的分子工具很重要。在这里，我们报道了一系列新的环金属化铂（II）配合物，当与G四重DNA结合时，显示出磷光的增加，作为开关探针。更重要的是，在与G -四联体结合后，它们显示出选择性的和明显的发射寿命延长。我们表明，这种效应可以使用磷光寿命成像显微镜（PLIM）选择性地可视化细胞中的这些结构。
G‐quadruplex DNA is a non‐canonical structure that forms in guanine‐rich regions of the genome. There is increasing evidence showing that G‐quadruplexes have important biological functions, and therefore molecular tools to visualise these structures are important. Herein we report on a series of new cyclometallated platinum(II) complexes which, upon binding to G‐quadruplex DNA, display an increase in their phosphorescence, acting as switch‐on probes. More importantly, upon binding to G‐quadruplexes they display a selective and distinct lengthening of their emission lifetime. We show that this effect can be used to selectively visualise these structures in cells using Phosphorescence Lifetime Imaging Microscopy (PLIM).